干扰素治疗对慢性乙肝患者细胞因子和抗病毒疗效的影响分析  

Effect of interferon on levels of cytokines and on result of anti-virus in patients with chronic hepatitis B

作　　者：蒋雪花[1] 孙永年[1] 黄祝青[1] 龚守军[1] 

机构地区：[1]武警上海总队医院传染科,上海201103

出　　处：《中国现代医学杂志》2004年第24期76-78,共3页China Journal of Modern Medicine

摘　　要：目的探讨血清细胞因子(IL-2,IL-10,IL-12和sIL-2R)在慢性乙型肝炎中的作用及抗病毒治疗对其水平的影响。方法采用ELISA方法检测干扰素治疗前、治疗6个月后的91例慢性乙肝患者血清IL-2,IL-10,IL-12和sIL-2R活性含量;HBV-DNA定量检测采用PCR方法。结果治疗前慢性乙肝患者血清IL-10和sIL-2R活性含量均明显高于对照组差异显著(P<0.05或P<0.01);而血清IL-2和IL-12在Ⅱ组(有效组)、Ⅰ组(无效组)及对照组间无差异性(P>0.05)。治疗后Ⅱ组血清IL-10和sIL-2R含量明显降低,与对照组无差异性(P>0.05);其血清IL-2和IL-12明显升高,同对照组、Ⅰ组相比差异显著(P<0.01)。结论IL-2,IL-10,IL-12和sIL-2R均参与慢性乙肝的发病机制,并可作为评估干扰素抗病毒治疗的重要免疫指标。Objective: To investigate the roles of interleukin-2, interleukin-10, interleukin-12 and interleukin-2 receptor in the development of chronic hepatitis B, and to study the effects of interferon on the levels of cytokines mentioned above. Methods: Before and after administration of interferon-αof 6 months , the levels of IL-2, IL-10, IL-12, sIL-2R in the serums of 91 patients with chronic hepatitis B were detected by ELISA, HBV-DNA were determined by quantity PCR. Result: The levels of IL-10, sIL-2R of patients with chronic hepatitis B were higher than that of normal control group (P <0.05 or P <0.01), and the difference of IL-2, IL-12 levels betweenⅠgroup, Ⅱgroup and normal group is not significant pretreatment (P >0.05). After anti-virus therapy with interferon-α, the levels of IL-10,sIL-2R decreased significantly in the Ⅱ group, but there is no significant difference betweenⅡgroup and normal group (P >0.05). The levels of IL-2, IL-12 increased obviously in the Ⅱ group, significant difference were obviously between Ⅱgroup, Ⅰgroup and normal group(P <0.01). Conclusion: IL-2, IL-10, IL-12, sIL-2R participate in the development of chronic hepatitis B; and they are candidate markers of evaluating the anti-virus effect of interferon on immunity.

关 键 词：慢性乙肝 干扰素 IL-2 IL-10 IL-12 SIL-2R 

分 类 号：R512.62[医药卫生—内科学]