石榴叶提取物中鞣花酸在大鼠体内药物动力学研究  

Pharmacokinetic study of ellagic acid in rat after oral administration of pomegranate leaf extract

作　　者：雷帆[1] 邢东明 向兰 何希辉[1] 王卫华 钟睒睒 杜力军[3] 

机构地区：[1]中国医学科学院.中国协和医科大学药用植物研究所,北京100094 [2]海南养生堂药业股份有限公司,海口570216 [3]清华大学生物科学与技术系药物药理研究室,北京100084

出　　处：《药品评价》2005年第1期38-41,共4页Drug Evaluation

摘　　要：目的通过大鼠口服石榴叶提取物,研究其中活性成分鞣花酸的药动学特征。方法采用反相HPLC法,紫外检测,应用药动学软件3p87拟合大鼠体内鞣花酸的药动学模型。结果鞣花酸在大鼠体内呈二室模型分布,主要药动学参数为:α=1.07h-1,β=0.12h-1,Ka=5.67h-1,t1/2α=0.65h;t1/2β=5.94h;t1/2Ka=0.12h;K21=0.36h-1;K10=0.35h-1;K12=0.48h-1;AUC=0.85g·h·L-1。结论鞣花酸药动学特征为口服血药浓度低,大部分经胃吸收,达峰时间短,快速吸收、快速分布消除。石榴叶中鞣花酸的吸收大于鞣花酸单体的吸收。Objective To study on pharmacokinetic character of ellagic acid through rat that has pomegrante leaf extract. Methods Quantification of ellagic acid, the principal bioactive component of pomegranate leaf extract, in rats plasma following oral administration of pomegranate leaf extract was achieved by using a high-performance liquid chromatographic method. Results The calibration curve for ellagic acid was linear (r2=0.9998) over the concentration range 26～1300ng/ml. The coefficients of variation of intra- and inter-day assays were 1.79%, 1.3%, 0.69%, 0.54% and 2.68%, 3.35%, 4.59%, 6.52% at concentrations of 26, 65, 130,1300 ng/ml, respectively. The recoveries of ellagic acid from rat plasma were found to be 94.5, 102.4, 101.9 and 106% for concentrations of 26, 65, 130, 1300 ng/ml, respectively. The concentration-time profiles was fitted with an open two-compartment system with lag time and the max concentration of ellagic acid in plasma was 213ng/ml only 0.55h after oral administration extract 0.8g/kg. Conclusion The pharmacokinetic profile indicates that ellagic acid has rapid absorption and elimination after oral administration pomegranate leaf extract, and part of it was absorbed from stomach.

关 键 词：鞣花酸 药代动力学 石榴叶提取物 口服 药动学模型 

分 类 号：R285[医药卫生—中药学]