具有GCSF活性的噬菌体展示肽  

Phage-displayed Peptides as Mimetics of Granulocyte Colony-stimulating Factor

作　　者：张力群[1] 王清明[1] 范国才[1] 陈吉中[1] 陈惠鹏[2] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]军事医学科学院生物工程研究所,北京100071

出　　处：《中国生物化学与分子生物学报》2004年第6期768-772,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金资助项目 (No .3 970 0 183 )~~

摘　　要：从NFS 6 0细胞中克隆了小鼠粒细胞集落刺激因子 (granulocytecolony stimulatingfactor,G CSF)受体的细胞因子受体同源区 (cytokinereceptorhomologous ,CRH)结构域 ,采用GST融合表达策略 ,实现了CRH结构域的表达 .以纯化的GST CRH融合蛋白为靶 ,从噬菌体随机环七肽库中筛选CRH结构域的结合肽 ,找到两组具有核心序列的噬菌体展示肽 .其中C2和C13噬菌体展示肽能刺激NFS 6 0细胞增殖 ,说明C2和C13噬菌体展示肽具有GGranulocyte colony-stimulating factor(G-CSF) is a cytokine that plays an essential role in maintaining the number of neutrophilic granulocytes in peripheral blood and is responsible for granulocytosis during inflammation. The cDNA encoding CRH domain of murine G-CSF receptor was amplified from NFS-60 cells, and was expressed in E.coli as GST-CRH fusion protein. The purified GST-CRH fusion protein was used as the target to screen a phage-displayed and disulfide-constrained heptapeptide library for CRH-binding peptide. Two groups of phage-displayed peptides with highly conserved motif were selected and analyzed, of which C2 and C13 could stimulate the proliferation of G-CSF-dependent NFS-60 cells, indicating that C2 and C13 were mimetics of G-CSF.

关 键 词：粒细胞集落刺激因子 细胞因子受体同源区结构域 噬菌体展示肽 肽库 

分 类 号：Q78[生物学—分子生物学]