^(99)Tc^mTRODAT-1多巴胺转运蛋白SPECT脑显像在亚临床期和临床期帕金森病模型猴诊断中的研究  

SPECT imaging of dopamine transporters in subclinical and clinical hemi parkinsonism monkeys with ^(99 )Tc ~m TRODAT-1

作　　者：边艳珠[1] 刘怀军[2] 张锦明[3] 李金福[1] 张文艳[1] 田国章[1] 魏强[1] 

机构地区：[1]河北省人民医院核医学科,河北石家庄050051 [2]河北医科大学第二医院医学影像科 [3]中国人民解放军总医院核医学科

出　　处：《中国医学影像技术》2004年第12期1832-1835,共4页Chinese Journal of Medical Imaging Technology

摘　　要：目的　探讨99Tcm TRODAT 1多巴胺转运蛋白SPECT脑显像在帕金森病亚临床期诊断中的价值。方法　对 4只食蟹猴静脉注射多巴胺转运蛋白显像剂99Tcm TRODAT 1,观察99Tcm TRODAT 1在不同时间点正常猴体内分布并进行纹状体DAT功能分析。用MPTP分别制备无症状亚临床期和临床期PD猴模型 ,利用计算机感兴趣区 (ROI)技术进行纹状体DAT功能半定量分析。结果　99Tcm TRODAT 1最佳SPECT脑显像时间为注射99Tcm TRODAT 1后 3～ 4h。PD猴毁损侧 (右侧 )纹状体在亚临床期就已存在DAT功能的降低 ,并随着损伤的加重毁损侧纹状体DAT功能进一步降低 ,对侧纹状体的DAT也受损。结论　99Tcm TRODAT 1SPECT脑显像可用于PD的亚临床期诊断和病情监测。Objective To investigate the value of 99 Tc m TRODAT 1 DAT SPECT in diagnosing subclinical Parkinson's disease. Methods Four monkeys without auto rotation were recruited and marked as M1, M2, M3, M4, respectively. The whole body scan was done on M1 and M2 after 99 Tc m TRODAT 1 555 MBq injection. The radioactivity distribution ratio of each organ at various time points was calculated by region of interest (ROI) technique. 99 Tc m TRODAT 1 SPECT imaging were obtained on M1 and M2 at various time point after 99 Tc m TRODAT 1 555 740 MBq injection. Striatum/cerebellum radioactivity ratios were calculated by ROI technique on 4 transverse imaging. Striatum/cerebellum radioactivity ratio represents striatum DAT function. Subclinical hemi parkinsonism monkeys were founded on M1, M2, M3, M4 by given MPTP 0.3 mg/kg through right internal carotid artery. Clinical hemi parkinsonism monkeys were founded on M2, M3, M4 by given MPTP 0.7mg/kg through right internal carotid artery for second time. Subclinical and clinical hemi parkinsonism monkeys accepted 99 Tc m TRODAT 1 DAT SPECT, and striatum DAT functions were evaluated by ROI technique, respectively. Results The major radioactivity was excreted by the hepatobiliary system. The optimal imaging time for 99 Tc m TRODAT 1 SPECT was 3-4 hours after injection. In subclinical hemi parkinsonism monkeys, right striatum DAT function was lower than left (1.33±0.08, 1.59±0.07, P <0.01). In clinical hemi parkinsonism monkeys, right and left Striatum/cerebellum radioactivity ratios were 1.03±0.06, 1.12±0.07 ( P <0.01). Conclusion 99 Tc m TRODAT 1 DAT SPECT can explore the subclinical DAT function changes in hemi parkinsonism monkeys. 99 Tc m TRODAT 1 DAT SPECT is a useful method for early diagnosis of subclinical Parkinson's disease.

关 键 词：帕金森病 断层摄影术 正电子发射型 多巴胺 TRODAT一1 动物模型 

分 类 号：Q95-33[生物学—动物学] R817[医药卫生—影像医学与核医学]