壳聚糖纳米颗粒包裹对鼠白细胞介素2基因表达和调节小鼠免疫效应的影响  

Effects of Entrapment of Murine Interleukin-2 Gene with Chitosan Nanoparticles on Expression of mIL-2 Gene and on Regulation of Imm une Response in Mice

作　　者：李惠[1] 高荣[1] 武梅[1] 付满良[1] 吴凯源[1] 杨毅[1] 王秀英[1] 王泽洲[1] 刘世贵[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《生物医学工程学杂志》2004年第6期947-952,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 (3 0 170 70 3 )

摘　　要：本实验制备壳聚糖纳米颗粒 (CNP) ,包裹小鼠白细胞介素 2基因 (mIL 2 )真核表达质粒 (VRMIL 2 ) ,肌注接种 2 1d昆明小鼠 ,观察mIL 2基因体内表达及其对免疫应答和免疫保护的影响。实验结果发现 :CNP包裹VRMIL 2注射小鼠血液中IgG、IgM和IgA不同程度地增多 ,均显著高于CNP包裹空白质粒组 (P <0 .0 5 ) ;其血清中IL 2、IL 4和IL 6的含量明显升高 ,与对照组差异显著 (P <0 .0 5 ) ;外周血液的白细胞和淋巴细胞数量也较对照组显著增加。免疫后 35d以大肠杆菌口服攻毒实验小鼠 ,检测发现 :CNP包裹VRMIL 2组小鼠的上述免疫指标除中性粒细胞外均显著多于对照小鼠 ,VRMIL 2接种小鼠均健康存活 ,而对照小鼠均发病 ;尽管CNP包裹VRMIL 2接种小鼠的体液和细胞免疫指标与未包裹VRMIL 2免疫鼠差异不显著 (P >0 .0 5 ) ,但剂量仅为后者的 1/ 5。这些结果表明 :壳聚糖纳米颗粒包裹VRMIL 2可显著提高外源IL 2基因体内表达水平 ,明显增强体液和细胞免疫水平的效应 ,增强对大肠杆菌的抗病力 ,提示壳聚糖纳米颗粒包裹IL 2基因可明显增强动物的体液和细胞免疫 ,可作为有效的抗感染免疫调节剂。The experiment was conducted to prepare chitosan nanopar ti cles (CNP), to entrap VRMIL-2 with CNP, the eukaryotic VR1020 expression plasmi d containing murineIL-2 gene(mIL-2), and to investigate the expression in vivo and the regulatory effect of mIL-2 on immune-response and immuno-prote ction in mice inoculated muscularly with CNP entrapped VMIL-2 at 21 days old. T he results showed that IgG, IgM and IgA contents increased to different degrees in the sera from the inoculated mice, which were remarkably higher than those of the controls inoculated VR1020 packed with CNP (P<0.05); so were the IL-2 ,IL-4 and IL-6 contents in the sera of the immunized mice. The number of whit e blood cells and lymphocytes significantly increased respectively in the vaccin ated mice, compared with those of controls. These mice were orally challenged wi th virulent E.coli 35 days post-inoculation, and all the immune responses were significantly higher than those of the control except the number of neutrophils. The mice inoculated with VRMIL-2 survived healthily, while the mice of control group were ill with the evident lesions. Although there are no remarkable diffe rences between the cellular and humoral immune indexes of mice inoculated with C NP-VRMIL-2 and nude VRMIL-2 (P>0.05), the dosage of CNP-VRMIL-2 is only one fifth of the VRMIL-2. These indicated that entrapment of mIL-2 gene with chitosan nanoparticles could remarkably enhance the expression of mIL-2 in vi vo, and significantly raise the levels of cellular and humoral immune, and inc rease the resistance of mice against E.coli infection. The results suggested tha t chitosan nanoparticles and IL-2 gene could be used as an effective immunoenha ncer to increase the immunity of animals against infection.

关 键 词：小鼠 壳聚糖纳米颗粒 CNP 白细胞介素2 细胞免疫 体液 IL-2基因 包裹 增强 调节剂 

分 类 号：R392[医药卫生—免疫学] R780.2[医药卫生—基础医学]