残耳软骨细胞种植于聚羟基丁酸酯-聚羟基己酸酯共聚物形成组织工程软骨  

Tissue engineered neocartilage using polymer substrates and chondrocytes rrom residual ear

作　　者：金骥[1] 庄洪兴[1] 胡平[2] 

机构地区：[1]中国医学科学院中国协和医科大学整形外科医院,北京100041 [2]清华大学化工系

出　　处：《中华医学美学美容杂志》2004年第6期352-355,共4页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的　探讨以聚羟基丁酸酯 聚羟基己酸酯 (PHB PHH)共聚物为细胞外支架、以残耳软骨作为种子细胞形成组织工程软骨的可能性。方法　取先天性小耳畸形 8例患者的残耳软骨 ,以胶原酶消化后种植于PHB PHH支架 ,体外培养 1周后种植于 8只裸鼠一侧背部皮下为实验组 ,另一侧只植入支架材料作为对照组。于 4周、8周后取出标本 ,做大体观察及HE染色、Masson三色染色检查。结果　 4周时实验组镜下显示有新生软骨形成 ,但仍有部分支架材料残留 ;8周时实验组标本大体观察及HE染色、Masson三色染色检查新生软骨与人耳软骨相似 ,支架材料已完全吸收。对照组无软骨形成。结论　以残耳软骨作为种子细胞 ,以PHB PHH共聚物为细胞外支架可以形成组织工程软骨 ,新生软骨大体观察、组织学检查与人耳软骨相似。Objective To investigate the possibility of using poly-hydroxybutyate-hydroxyhexate p(3HB-co-3HH)), a modified extracellular matrix, the third generation of PHA family, as a scaffold for seeding of chondrocytes from the residual ear of microtia patients to generate tissue engineered cartilage in athymus mice. Methods The residual ear cartilage from eight 7-years-old microtia patients was enzymatically dissolved by collagenase, and chondrocytes were harvested and seeded into foams of PHB-PHH. After incubation for 1 week in vitro, chondrocyte-polymer constructs were implanted subcutaneously into 8 athymus mice. A control groups was established by subcutaneous implantation of PHB-PHH foams alone. One athymus mice were killed at 4 weeks postoperatively, and 6 were sacrificed at 8 weeks. The specimens were dissected and examined macroscopically and histologically. Results Specimens harvested from chondrocyte-polymer constructs subjected to gross morphologic and histology analysis demonstrated new cartilage formation, and those from control groups showed no cartilage formation. The one of 4 weeks still had some remains of the scaffold with nodules of neocartilage. After 8 weeks, all the 6 mice had neocartilage formed almost the same as natural. The PHB-PHH scaffold were totally absorbed. Conclusion These findings demonstrate that the foam of PHB-PHH is not only a good 'matrix' for cartilage tissue engineering, but also optimal scaffold for the seeding of chondrocytes from the residual ear to generate new cartilage that would be useful in plastic and reconstructive surgery. Chondrocytes from the residual ear of microtia patients are good candidates for generation of tissue engineered cartilage.

关 键 词：小耳畸形 软骨 组织工程 聚羟基丁酸酯-聚羟基己酸酯 细胞外支架 

分 类 号：R318.08[医药卫生—生物医学工程]