人外周血白细胞端粒DNA长度变化与性别的关系  

Gender specific profiles of human telomere shortening during aging

作　　者：葛璐璐[1] 刘超[1] 陈晓晖[1] 陈向红[1] 陶黎阳[2] 赵永忠[3] 陈平雁[4] 

机构地区：[1]广州市刑事科学技术研究所,广东广州510030 [2]中山大学基础医学院法医物证学教研室,广东广州510089 [3]第一军医大学细胞生物教研室,广东广州510515 [4]第一军医大学医学统计学教研室,广东广州510515

出　　处：《中国法医学杂志》2004年第6期337-339,共3页Chinese Journal of Forensic Medicine

基　　金：公安部科技攻关资助项目(994421484)

摘　　要：目的 探讨人外周血白细胞端粒DNA长度变化与性别的关系。方法 选取123例不同年龄健康人外周血样本，其中男性63例，女性60例，酚氯仿法抽提人基因组DNA，采用地高辛标记探针，通过Southern Blot方法检测端粒DNA限制性酶切片段(TRF)长度的变化。结果 123例外周血白细胞端粒TRF平均长度随年龄增长而逐渐缩短，TRF随年龄缩短的速度是不均一的，且呈现出性别差异。结论 人外周血白细胞端粒DNA长度随年龄缩短的变化速率具有性别差异，通过端粒DNA长度推断个体年龄需考虑到性别因素。Objective In human,both in vivo and in vitro,telomere shortening appears to be a major component of cell senescence and aging. However, gender specific human telomere shortening needs to be further characterized. Therefore our study is aimed at clarifying gender-dependent profiles of telomere shortening. Methods 123 peripheral blood samples were collected from healthy individuals of different ages. The mean telomeric restricted fragment (TRF) was measured using Southern Blotting with Dig-labeled probe. Results Distinguished dynamics profiles of telomere shortening were observed among different age groups. Conclusion The result indicates that there are gender specific dynamic profiles of telomere shortening. Therefore, the gender must be considered when an individual age is estimated by telomeric restricted fragment length assay.

关 键 词：人外周血 外周血白细胞 性别 年龄增长 不同年龄 人基因组 血样本 端粒DNA 限制性酶切 氯仿法 

分 类 号：D919[医药卫生—法医学]