大鼠电击死心脑肺及皮肤超微结构的改变  

A study on the ultrastructural changes of the heart, brain, lung and skin in the electrocuted rats

作　　者：竞花兰[1] 姜富学[1] 成建定[1] 张有宾[1] 张宜骏[1] 李章旺[2] 吴金浪[2] 

机构地区：[1]中山大学基础医学院法医学系,广东广州510089 [2]中山大学基础医学院电镜室,广东广州510089

出　　处：《中国法医学杂志》2004年第6期344-346,共3页Chinese Journal of Forensic Medicine

摘　　要：目的 观察电击致死大鼠的心、脑、肺、皮肤超微结构形态学改变，寻找电击死的法医学鉴定依据。方法 大鼠12只，其中电击致死6只，另6只对照；取心、脑、肺、皮肤，用戊二醛固定、锇酸染色后透射电镜观察。结果 所取组织细胞均见明显细胞凋亡样改变，其中红细胞肿胀变形，充填整个毛细血管管腔。结论 心、脑、肺、皮肤组织细胞与血管内皮细胞的超微结构形态改变对鉴定电流死有参考价值。Objective To detect the morphological evidence of identifying death from electrical injury, the ultrastructural changes of the heart, brain,lung and skin in the electrocuted rats were observed by transmission electron microscopy (TEM). Methods 12 rats were divided into the experimental and control groups. In the experimental group,6 rats were killed by electrocution, and in the control group, 6 rats were killed by decapitation. Samples from the heart, brain, lungs, and skin were fixed with valeric aldehyde and post-fixed with osmic acid. After being stained, the ultrathin sections were observed by TEM. Results Evident cell apoptosis was detected in all the tissues examined, and erythrocytes appeared swelling, which stuffed the lumen of the capillary vessels. Conclusion The ultrastructural changes of the cells of the heart, brains, lungs, and skins including the vascular endothelial cells may be used as the indicators for identifying death from electrical injury.

关 键 词：法医病理学 电击死 细胞形态 超微结构 

分 类 号：D919[医药卫生—法医学]