氟乙酸五氟苄基酯的气相色谱分析  

Gas chromatographic analysis of 2,3,4,5,6-Pentafluorobenzyl monofluoroacetate

作　　者：施文兵[1] 谢珍茗[1] 刘岚[1] 桑向玲 裴茂清[2] 赵楚云[2] 刘卫国[2] 邓芹英[1] 

机构地区：[1]中山大学化学与化学工程学院,广东广州510275 [2]广东省公安厅,广东广州510050

出　　处：《中国法医学杂志》2004年第6期354-356,共3页Chinese Journal of Forensic Medicine

基　　金：广东省科技计划项目(2001;c32202)广东省公安厅科研基金(2001)

摘　　要：目的 对氟乙酸根阴离子的衍生物-氟乙酸五氟苄基酯(PFB-MFA)进行了气相色谱分析，以满足氟乙酰胺类鼠药化验工作的需要。方法 使用GC／ECD、GC／MS Scan、GC／MS SIM对该化合物的标准溶液进行了分析，得到了各种分析方法的校正曲线及检测限。结果 三种方法测定PFB-MFA的线性范围分别为GC／ECD 0.01～0.1ng／μl、GC／MS SCAN 1～100ng／μl、GC／MS SIM 5×10^(-3)～1ng／μl。三种方法对氟乙酸根阴离子的检测限分别为：GC／ECD1.31×10^(-4)ng／μl、GC／MS SCAN 0.13ng／μl、GC／MS SIM 1.76×10^(-4)ng／μl。结论 氟乙酸根阴离子的衍生物氟乙酸五氟苄基酯在GC／ECD及GC／MS上具有一定的线性范围，有非常高的灵敏度，可用于法庭科学的定性定量分析。Objective To determine 2, 3, 4, 5, 6-pentafluorobenzyl monofluoroacetate (PFB-MFA) as the derivative of monofluoroacetate anion ion by gas chromatography, by which fluoroacetamide, one of the raticides may be analyzed. Methods The calibration curve and limit of detection were obtained by quantification of standardized PFB-MFA using GC/ECD and GC/MS. Results The linear ranges for PFB-MFA were 0.01～0.1ng/μfor GC/ECD,1～100ng/μl for GC/MS SCAN and 5×10^(-3)～1 ng/μl for GC/MS SIM. The limit of detections for fluoroacetate anoin were 1.31×10^(-4)ng/μl for GC/ECD, 0.13 ng/μl for GC/MS SCAN, and 1.76×10^(-4) ng/μl for GC/MS SIM. Conclusion The monofluoroacetate anion derivative (MFAPFB) can be deterimined accurately with high sensitivity by GC/ECD and GC/MS SIM, which may be used for analysis of fluoroacetamide in forensic practice.

关 键 词：GC/MS 检测限 MFA 氟乙酰胺 化验 鼠药 定性定量分析 GC/ECD 法庭科学 目的 

分 类 号：D919[医药卫生—法医学]