铈对人膀胱癌细胞基质金属蛋白酶-9表达及活性的影响  

Effect of Cerium on Expression and Activity of MMP-9 from Human Carcinoma of Bladder Cell Line

作　　者：李瑾 胡国武[1] 欧阳砥[1] 牛瑞芳[2] 周永洽[1] 申泮文[1] 

机构地区：[1]南开大学化学系,天津300071 [2]天津肿瘤医院中心实验室,天津300060

出　　处：《中国稀土学报》2003年第1期85-88,共4页Journal of the Chinese Society of Rare Earths

基　　金：国家九五攻关课题资助项目(96 906 01 13)

摘　　要：以体外培养的人膀胱癌细胞为研究对象,采用四唑盐比色检测法、明胶酶谱法研究Ce4+对人膀胱癌细胞的存活、基质金属蛋白酶 9表达与活性的影响。结果表明,低浓度稀土(0 01mmol·L-1)对细胞的生长没有显著影响,但强烈抑制基质金属蛋白酶 9的表达,而此浓度Ce4+能提高基质金属蛋白酶 9的活性。高浓度稀土(1 0mmol·L-1)对细胞的生长、基质金属蛋白酶 9表达与活性均有显著的抑制作用。The effect of Ce4+ on cell growth, expression and activity of matrix metalloproteinase9 (MMP9) with MTT colorimetry and gelatin Zymography assays in human bladder carcinoma cell line was investigated The results indicate that the lower concentration of Ce4+ (001 mmol·L-1) has no influence on cell growth, but inhibits extremely expression of MMP9 and increases its activity, whereas the higher concentration of Ce4+ (10 mmol·L-1) can inhibit all of them The results raise the possibility that Ce4+ may have beneficial effects in attenuating invasion and metastasis of malignant tumors

关 键 词：生物化学 明胶酶谱法 基质金属蛋白酶 恶性肿瘤 稀土 

分 类 号：O614.33[理学—无机化学] Q556[理学—化学]