卡托普利和氯沙坦对关木通所致大鼠肾间质纤维化的防治作用  

Prophylactico-therapeutic Effect of Captopril and Losartan on Interstitial Fibrosis of the Rat Kidney Induced by Guanmutong

作　　者：朱少铭[1] 刘久波[1] 陈琳[1] 李艳[1] 姚俊霞[1] 金朝霞[1] 王作军[1] 吴平勇[1] 罗强[1] 周娟[1] 

机构地区：[1]湖北省郧阳医学院附属太和医院肾内科,十堰442000

出　　处：《医药导报》2005年第2期112-115,共4页Herald of Medicine

摘　　要：目的　探讨卡托普利和氯沙坦对关木通所致慢性肾间质纤维化的防治作用 ,确定肾纤维化的检测指标 ,为临床诊治肾纤维化提供依据。方法　将 2 4只健康雄性大白鼠随机分成 4组 ,每组 6只。对照组正常饲养观察 ;模型组以生药浓度为 2 g·mL 1 的关木通水煎液灌胃 ,6g·kg 1 ·d 1 ,qd ;实验组 1在模型组给药基础上以 0 .5 1mg·mL 1 卡托普利悬液灌胃 ,1.66mg·kg 1 ·d 1 ,qd ;实验组 2在模型组给药基础上以 0 .2 5mg·mL 1 氯沙坦悬液灌胃 ,0 .83mg·kg 1 ·d 1 ,qd。实验开始前和实验第 2 ,4,6周自大鼠内眦静脉采血 ,测定血生化、肾功能及放射免疫指标 ,实验第 42天从心脏直接采血后处死大鼠 ,取肾脏作组织形态学和特殊染色检查。结果　给药后模型组、实验组 1、实验组 2的肾功能和血生化指标明显偏离正常范围。实验第 4,6周 ,模型组血尿素氮 (BUN)、血肌酐 (Scr)与本组实验开始前有明显差异 (P <0 .0 1) ;实验组 1、实验组 2的BUN、Scr与同时期模型组有明显差异 ,均P <0 .0 1,前两者检测指标更接近正常范围。模型组、实验组 1、实验组 2放射免疫指标均偏离正常范围 ,但后两组更接近正常值 ,3组动物肾脏均有纤维化改变 ,其中模型组、实验组 2纤维化程度更严重。Objective To probe into the prophylactico-therapeutic effect of captopril and losartan on chronic interstitial renal fibrosis induced by guanmutong in the rat and define the indices for detection of renal fibrosis so as to provide experimental basis for the clinical diagnosis and treatment of renal fibrosis. Methods 24 adult male Wistar rats were randomly divided into 4 equal groups. Rats of the control group were bred normally for comparison. Rats of the model group were given each once a day by gastrogavage 6 g·kg -1 of guanmutong in a decoction with a concentration of 2 g·mL -1 of the crude drug. On the basis of administration of guanmutong in the same manner as that described for animals of the model group, rats of the test group Ⅰand test group Ⅱ were given each once a day additionally by gastrogavage 1.66 mg·kg -1 of captopril in a suspension with a concentration of 0.51 mg·mL -1 of the drug and 0.83 mg·kg -1 of losartan in a suspension with a concentration of 0.25 mg·mL -1 of the drug, respectively. Venous blood was taken from behind the inner canthus of the animals before and 2,4,6 weeks after the beginning of the experiment for the determination of blood chemistry, renal function and radioimmunological indices, respectively. On day 42 of the experiment, blood was taken from the heart and the animals were then sacrificed. The kidney was removed for histological examination and special staining . Results Indices of renal function and blood chemistry were evidently deviated from the normal range in animals of the model group ,test group Ⅰand test group Ⅱafter guanmutong administration. Levels of blood urea nitrogen (BUN) and serum creatinine(Scr) in rats of the model group in the 4th and 6th week were much higher than those before the beginning of the experiment(P<0.01). Levels of BUN and Scr in animals of the test group Ⅰand test group Ⅱ, although higher than normal, were apparently lower than those of the rats of the model group at the same time points, (P<0.01).The radioim

关 键 词：马兜铃酸 肾间质纤维化 

分 类 号：R972.4[医药卫生—药品] R965[医药卫生—药学]