壳聚糖DCX-16的初步安全性评价  

Elementary Evaluation of the Safety of Chitosan DCX-16

作　　者：曾健[1,2] 李晓辉[1] 黄钺华[1] 张海港[1] 李淑慧[1] 

机构地区：[1]第三军医大学基础部 [2]解放军第254医院,天津市300142

出　　处：《中国药房》2005年第3期172-174,共3页China Pharmacy

基　　金：国家自然科学基金资助项目 (5033020)

摘　　要：目的 :初步评价新壳聚糖衍生物DCX -16的安全性。方法 :通过对小鼠腹腔注射壳聚糖DCX -16后进行耐量试验、家兔眼刺激性及肌肉刺激性试验 ,以及用噻唑蓝比色法观察壳聚糖DCX -16对体外培养的3T3成纤维细胞增殖的影响等研究 ,评价壳聚糖DCX -16的安全性。结果 :小鼠对壳聚糖DCX -16的日最大耐受量为3. 0g/kg ;壳聚糖DCX -16对家兔眼睛及肌肉均无刺激性作用 ;3T3细胞经壳聚糖DCX -16作用后生长良好 ,增殖率在第2、3、4d时与对照组无显著性差异。结论 :新型壳聚糖DCX -16具有一定的安全性。OBJECTIVE:To evaluate the safety of Chitosan DCX-16elementarily.METHODS:DCX-16was injected i.p.to observe the acute toxicity in mice.The local irritating effects were observed on tolerance test,cerato-conjunctiva and muscles stimulus test in rabbits.The cell shape and proliferative rate of3T3cells were determinated by MTT in cell culture with DCX-16.RESULTS:It showed that DCX-16had no irritation on the eyes and muscles in rabbits.Tolerance dose of DCX-16in mice was as high as3.0g/kg per day.Cell culture with DCX-16demonstrated that3T3cell's shape and growth were normal.The relative growth rate of3T3cells had no statistical difference between control and DCX-16groups on the2nd,3rd,4th day.CONCLUSION:DCX-16is safe.

关 键 词：壳聚糖DCX-16 细胞毒性 局部刺激作用 3T3细胞 

分 类 号：R965.3[医药卫生—药理学]