泡沫分离对甘草酸的纯化和富集  

Purification and Enrichment of Glycyrrhizin by Foam Fractionation

作　　者：傅博强[1] 李欢[1] 刘劼[1] 李磊[2] 王小如[1,2] 黎先春[2] 

机构地区：[1]厦门大学化学化工学院化学系现代分析科学教育部重点实验室,福建厦门361005 [2]国家海洋局第一研究所环境与生命过程中物质迁移与反应机理研究室,山东青岛266061

出　　处：《现代中药研究与实践》2004年第B12期57-61,共5页Research and Practice on Chinese Medicines

摘　　要：本方法采用泡沫分离对甘草酸的混合物进行纯化富集。以富集比、质量回收率以及泡沫液中甘草酸的HPLC光谱纯度为纯化富集效果的表征参数。考察了氮气流量、甘草酸的初始进料浓度、原料液的pH值以及泡沫分离柱的尺寸对纯化富集效果的影响。结果表明富集比随参数不同在3．35～12．8之间变化，随氮气流量、甘草酸的初始进料浓度的降低而增加，适度增加泡沫分离柱的高度和内径也会使富集比变大。质量回收率最高达91．7％，随氮气流量、甘草酸的初始进料浓度、泡沫分离柱的高度和内径的增加而增大。泡沫分离所得甘草酸的质量纯度和HPLC光谱纯度分别为82．4％和90．2％，而对原料甘草酸单铵盐不纯物则分别为76．0％和86．0％。结果表明，泡沫分离纯化富集甘草酸省时，省力，成本低。同时，本研究为此方法的工业化提供了一些指导。A foam fractionation method was employed to purify and enrich a mixture of glycyrrhizin (GL) from licorice extracts. The performance of the technique was evaluated using the criteria of enrichment ratio, mass recovery and HPLC chromatographic purity of GL in foamate. The effects of the operational factors, i.e. nitrogen gas flow rate, initial feed GL concentration, pH of raw solution and the size of the foaming column on separation have been investigated. The results show that the enrichment ratio ranges 3. 35-12. 8, and increases with decreasing nitrogen gas flow rate, decreasing feed GL concentration, and increases moderately with the height and inside diameter of column. The mass recovery of GL can be as high as 91. 7% and increases with increasing nitrogen flow rate, increasing feed GL concentration and increasing dimensions of column. The mass purity and the HPLC purity of the GL powder obtained by foam fractionation are 82. 4% and 90. 2%, respectively, comparing with the raw GL powder of 76. 0% and 86. 0% , respectively. The results show that foam fractionation is cost-effective for the purification and enrichment of GL. The study provides useful technical guidelines for its further up-scaling for industrial applications.

关 键 词：甘草酸单铵盐 纯化 HPLC 泡沫分离 效果 增加 浓度 进料 富集 氮气 

分 类 号：R284[医药卫生—中药学] TQ028[医药卫生—中医学]