外源性卵黄蛋白对鳗鲡GTH细胞和卵母细胞超微结构的影响  

EFFECTS OF EXOGENOUS YOLK PROTEIN ON THE ULTRASTRUCTURES OF GONADOTROPIC CELL AND OOCYTE IN ELL (ANGUILLA JAPONICA)

作　　者：姜仁良[1] 吴嘉敏[1] 高兵[1] 

机构地区：[1]上海水产大学渔业学院,200090

出　　处：《上海水产大学学报》1994年第3期98-103,共6页Journal of Shanghai Fisheries University

摘　　要：外源性卵黄蛋白与鲤鱼垂体（CPG）加绒毛膜促性腺激素（HCG）混合注射（试验组）对鳗鲡具有明显的催热作用，卵巢成热系数平均为56．17±11．74％，是对照组（CPG加HCG组）的2．87倍。试验组脑垂体促性腺激素细胞（GTH细胞）中除含有数量较多的小分泌颗粒外，球状分泌颗粒增加，内质网池扩大；卵母细胞直径为450．0微米，双层滤泡膜和放射带充分发育。对照组GTH细胞的球状分泌颗粒直径和内质网扩大均小于试验组；卵母细胞直径为270．0微米。而饲养在海水中75天，未经注射催情药物的雌鳗，脑垂体GTH细胞仅有小分泌颗粒，未见球状分泌颗粒，卵母细胞的放射带正在形成，胞内开始出现脂肪油球。试验结果表明，外源性卵黄蛋白与CPG加HCG混合注射有明显协同作用，对卵母细胞成热有显著促进作用。A intraperitoneal injection with exogenous yolk protein combining with carp pituitary gland (CPG) and human chorionic gonadotropin (HCG (were given to eel (Anguilla japonica) periodically. The gonadosomatic index (GSI) of treated (group reached to 56.17±11. 74 %; it was 2. 87 times of the control group (CPG+HCG). Both small secretory granules and globular secretory granules and endoplasmic reticulum enlargements were showed in gonadotropic cells (GTH cells) of treated eel. The average diameter of oocyte was 450.0μm,two layers of folliclar rnembrane and radiate zone were well developed. Both the diameter of globular secretory granu1es and endoplasmic reticulum enlargement in control group were smaller than that of treated group and the average diameter of oocyte was 270.0μm. While the eels were reared in seawater for 75 days without any injection, it was only small secretory granules appearance in GTH cells; the radiate zone of oocyte has not formed,some lipid globules appeared in oocyte cytoplasma, These results indicated that the exogenous yolk protein has a synergetic effects with CPG and HCG mixed injection and it shows a significantly promotive effects in oocyte maturation of eel.

关 键 词：分泌 外源性 CPG 对照组 卵母细胞 放射 脑垂体 卵黄蛋白 鳗鲡 催情 

分 类 号：S965.223[农业科学—水产养殖] S881.2[农业科学—水产科学]