尿液中内皮素-1在兔不全梗阻性膀胱中的研究  

Changes of urine endothelin in rabbit bladder outlet obstruction

作　　者：程文[1] 周四维[2] 高建平[1] 张征宇[1] 葛京平[1] 周文泉[1] 马宏青[1] 周水根[1] 魏武[1] 薛松[1] 梁培禾[1] 李汉平[1] 蔡荣星[1] 

机构地区：[1]南京军区南京总医院泌尿外科,江苏南京210002 [2]华中科技大学同济医学院附属同济医院泌尿外科,430030

出　　处：《医学研究生学报》2003年第11期808-810,共3页Journal of Medical Postgraduates

摘　　要：目的 :探讨尿液中内皮素 1(ET 1)在兔不全梗阻性膀胱中的变化和意义。　方法 :运用放射免疫分析技术对 10只成年雄性新西兰白兔不全梗阻性膀胱 (实验组 ,分为梗阻 3周组及梗阻 6周组 )及 10只同龄雄性新西兰白兔无梗阻性膀胱 (对照组 ,分为术后 3周组及术后 6周组 )兔尿液中ET 1进行研究。　结果 :尿液中ET 1含量实验组较对照组明显升高 ,实验组梗阻 3周组与对照组术后 3周组及实验组梗阻 6周组与对照组术后 6周组比较均差异显著 (P <0 .0 1) ,实验组梗阻 3周组与 6周组比无显著差异 (P >0 .5 ) ,对照组术后 3周组与术后 6周组比无显著差异 (P >0 .5 )。　结论 :在兔不全梗阻性膀胱中 ,ET 1对膀胱壁结构改变及功能紊乱具有一定作用 ,尿液中ET 1升高可作为诊断膀胱出口梗阻的一个重要参数。Objectives:To study changes and the significance of urine endothelin-1 in a rabbit model of partial bladder outlet obstruction. Methods: Rabbit model of partial bladder outlet obstruction was used to study changes of urine endothelin-1 with radioimmunoassay.Partial bladder outlet obstruction was induced in adult male New Zealand White rabbits(ten rabbits as experiment group,duration of obstruction was three weeks and six weeks).Sham operated age-matched rabbits acted as control(ten rabbits as control group,duration of postoperation were three weeks and six weeks). Results: Urine endothelin-1 of partial bladder outlet obstruction was significantly increased. Urinary obstruction for three weeks and six weeks caused significant elevation of urine endothelin-1 than controls (P<0.01). No differences were observed in the levels of endothelin-1 in the two experimental groups obstructing for three weeks and six weeks respectively (P>0.5). No differences were observed in the levels of endothelin-1 in the two control groups three weeks and six weeks postoperatively (P>0.5). Conclusions:The up-regulation of endothelin-1 was associated with altered bladder structure and dysfunction of rabbit partial bladder outlet obstruction, this was of diagnostic value for partial bladder outlet obstruction.

关 键 词：内皮素-1 尿液 兔不全梗阻性膀胱 

分 类 号：R694.2[医药卫生—泌尿科学]