ESN/AES-IGSS法与ELISA法检测双链DNA抗体的对照比较  

COMPARISON OF ESN/AES-IGSS METHODS WITH ELISA FOR DETECTING ANTI-dsDNA ANTIBODIES

作　　者：季晓辉[1] 袁淑云[1] 杜文平[1] 荣漪雯[1] 

机构地区：[1]徐州医学院微生物学教研室

出　　处：《徐州医学院学报》1993年第2期83-86,共4页Acta Academiae Medicinae Xuzhou

摘　　要：以ESN/AES-IGSS法与ELISA法相对照,检测了135份血清的抗双链DNA抗体。结果表明,ESN-IGSS、AES-肝-IGSS、AES-肾-IGSS与ELISA的符合率分别是91.1％、95.6％和95.6％。对SLE的敏感性ESN-IGSS为82％,在统计学意义上高于ELISA的68.9％(P<0.05);AES-肝/肾-IGSS均为73.8％,略高于ELISA,但无统计学意义(P>0.05)。对SLE的特异性,ESN-IGSS、AES-肝/肾-IGSS三法分别为91.8％、93.2％和93.2％,与ELISA的91.8％比较,差异无统计学意义(P>0.05)。因而进一步证明ESN/AES-IGSS法之可靠。Anti-dsDNA antibodies ( AdsDNA ) in 135 sera were tested by iramu -nogold silver staining (IGSS) with enzymes-treated human spermatozoal nuclei ( ESN ) or HCI-treated mouse liTer/kidney imprints ( AES-L/AES-K ) used as the substrate and simultaneously by,enzyme-linked immunosorbe-nt assay ( RLISA ) to compare the results obtained . The accordant rates of the results obtained from IGSS using ESN, AES-L and AES-K, with that obtained from ELISA were 91.1%, 95.6% and 95.6% respectively.To SLE , the sensitivity of IGSS was 82% when ESN was the substrate , significantly higher than that of ELISA (68.9%) ( P<0.05 ); but was 73.8% when AES-L or AES-K was nsed, slightly but not significantly higher than that of ELISA (P<0.05).The specificities of ESN-IGSS, AES-L-IGSS and AES-K-IGSS were 91.8%, 93.2% and 93.2% respectively, while that of ELISA was 91.8%, the differences not significant ( P>0.05). These results further show that the methods of ESN/AES-Igss for AdsDNA are reliable.

关 键 词：DNA 抗体 ELISA 免疫金银染色 

分 类 号：R446.61[医药卫生—诊断学]