In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane  

作　　者：NongYueHE JianXinTANG SongLI HongCHEN AnCunZHOU 

机构地区：[1]LaboratoryofNanotechnology,BiomedicineandDevices,ZhuzhouInstituteofTechnology,Zhuzhou412008 [2]LaboratoryofNanotechnology,BiomedicineandDevices,ZhuzhouInstituteofTechnology,Zhuzhou412008／／Chien-ShiungWuLaboratory,SoutheastUniversity,Nanjing210096 [3]LaboratoryofNanotechnology,BiomedicineandDevices,ZhuzhouInstituteofTechnology,Zhuzhou412008／／LifeScienceandTechnologyDepartmentofCentralSouthForestryUniversity,Changsha410004 [4]CollegeofHunanTraditionalChineseMedicine,Zhuzhou412000

出　　处：《Chinese Chemical Letters》2005年第3期397-400,共4页中国化学快报（英文版）

摘　　要：Polypropylene microporous membranes were treated with plasma in a mixture of N2 and H2 (1:2 in volume). Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FT1R), X-ray photoelectron spectroscopy (XPS) and ultra-violet (UV) spectra demonstrated the success of grafting amino groups. The density of the polar amino groups on the membrane surface is about 0.59 μmol/cm2. The as-treated membranes were successively applied to the in situ synthesis of oligonucleotides and an average coupling yield was more than 98%. The surface feature of the treated membrane is suggested to be responsible for its advantage over a glass slide.

关 键 词：PLASMA grafting. amino group in situ synthesis of oligonucleotides coupling efficiency polypropylene microporous membrane. 

分 类 号：O629.74[理学—有机化学]