Isolation and Characterization of a New Subspecies of Aeromonas salmonicida （ Aeromonas salmonicida subsp, flounderacida subsp. nov. ） from Stone Flounder（ Kareius bicoloratus L. ）  

作　　者：张晓君  Zhan Wenbin  Chen Cuizhen  Fang Hai  

机构地区：[1]Laboratory of Pathology and Immunology of Aquatic Animals, The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, P.R.China Department of Animal Science, Hebei Normal University of Science and Technology, Qinh [2]Laboratory of Pathology and Immunology of Aquatic Animals, The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, P.R.China [3]Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao 066600, P.R.China

出　　处：《High Technology Letters》2004年第4期87-93,共7页高技术通讯（英文版）

基　　金：国家高技术研究发展计划(863计划)，国家自然科学基金，国家重点基础研究发展计划(973计划)，河北省自然科学基金

摘　　要：Biological properties were studied to appropriate pathogenic bacteria which were isolated from di-seased (or dead) stone flounder (Kareius bicoloratus L.) which expressed bacterial septicaemia, including morphological characteristics, colony characteristics, physiological and biochemical characteristics and serum homology of isolates, the results showed that the isolates belonged to a new subspecies of A.salmonicida. In addition, the representative strains have been re-checked and detected the mol% G+C ratio of the DNA by China Center for Type Culture Collection (CCTCC), the examined strains were also regarded as a new subspecies of A.salmonicida, and designated as Aeromonas salmonicida subsp. flounderacida subsp.nov.by its isolated fish (Kareius bicoloratus). Molecular identification of analysis of the nucleotide sequence of the 16S rRNA gene were applied, the results showed high similarity (99%) with the 16S rRNA gene of Aeromonas salmonicida from GenBank database. Cluster analysis of phylogenetic tree revealed that the representative strain formed separately bootstrap-supported cluster.

关 键 词：石鲽 生物特征 病源体 RNA 比目鱼 

分 类 号：Q959.4[生物学—动物学]