Cytoplasmic male sterility of tuber mustard is associated with the alternative spliced mitochondrial T gene transcripts  

作　　者：PEIYanxi CHENZhujun CAOJiashu CHENXueiun LIUXiaohui 

机构地区：[1]CollegeofLifeScienceandTechnology,ShanxiUniversity,Taiyuan030006,China [2]CollegeofAgricultureandBiotechnology,ZhejiangUniversity,Hangzhou310029,China [3]KeyBiotechnologyLabofYunnanTobaccoScienceAcademy,Kunming650106,China

出　　处：《Chinese Science Bulletin》2004年第23期2481-2486,共6页

摘　　要：Two transcripts of T gene, T1170 and T1243, were obtained from the mitochondrial cDNA of tuber mus- tard CMS line. T1243 was a transcript with an intron unspliced, which has the basic characteristics of type Ⅱ intron. The expressions of the two transcripts were analyzed by reverse transcription PCR (RT-PCR). The results showed that, at seedling stage, the expression of T gene was mainly in the form of T1170 but decreased with the development gradually, while the expression abundance of another tran- script, T1243, increased gradually. The T1243 was prevalent at the profuse flowering stage. The expression pattern was confirmed by Northern blot analysis. These results suggested that the alternative spliced mitochondrial T gene transcripts were related to CMS of tuber mustard.Two transcripts of T gene, T1170 and T1243, were obtained from the mitochondrial cDNA of tuber mus- tard CMS line. T1243 was a transcript with an intron unspliced, which has the basic characteristics of type Ⅱ intron. The expressions of the two transcripts were analyzed by reverse transcription PCR (RT-PCR). The results showed that, at seedling stage, the expression of T gene was mainly in the form of T1170 but decreased with the development gradually, while the expression abundance of another tran- script, T1243, increased gradually. The T1243 was prevalent at the profuse flowering stage. The expression pattern was confirmed by Northern blot analysis. These results suggested that the alternative spliced mitochondrial T gene transcripts were related to CMS of tuber mustard.

关 键 词：雄性不育基因 细胞质 芥末 块茎 基因 DNA 

分 类 号：Q946[生物学—植物学] Q52