黄连解毒汤有效部位对一氧化氮诱导大鼠皮层神经元损伤的保护作用  

Protective Effect of Huanglian Jiedu Decoction Active Fraction on NO-induced Cerebral Cortical Neuron Injury

作　　者：吴彦 孙建宁[2] 石任兵[2] 

机构地区：[1]北京维健生生物科技有限公司,北京100039 [2]北京中医药大学中药药理系,北京100102

出　　处：《中国药科大学学报》2005年第1期59-62,共4页Journal of China Pharmaceutical University

摘　　要：目的 :观察黄连解毒汤有效部位 (HJDAF)对培养皮层神经细胞一氧化氮损伤的保护作用。方法 :体外培养新生大鼠皮层神经细胞 ,加入硝普钠观察其对神经细胞的损伤及HJDAF的保护作用。结果 :HJDAF(15 3mg/kg)可减少受损细胞的乳酸脱氢酶 (LDH)漏出率 ;6 10 ,30 5 ,15 3mg/kg可提高受损细胞的活性 ,减少丙二醛 (MDA)生成 (P <0 .0 5 ;P <0 .0 1) ;6 10mg/kg可提高受损细胞超氧化物歧化酶 (SOD)的活性。结论 :HJDAF对培养神经细胞NO损伤具有一定的保护作用 ,其机制可能与抗脂质过氧化作用有关。AIM:To investigate protective effects of Huanglian Jiedu Decoction active fraction(HJDAF) on NO-induced cerebral cortical neuron injury. METHOD:Cortical neuron cells of newly born rat were prepared cultured in vitro. The model of NO-induced cerebral cortical neuron injury was constructed.RESULT:HJDAF(153 mg/kg) reduced the leakage rate of lactic dehydrogenase(LDH);610,305,153 mg/kg increased the activity of neuron and reduced the production of maleic dialdehyde; 610 mg/kg increased the activity of SOD.CONCLUSION:HJDAF protects NO-induced cerebral cortical neuron injury by suppressing the generation of lipid peroxide.

关 键 词：黄连解毒汤有效部位 一氧化氮 皮层神经元 损伤 细胞培养 

分 类 号：R285[医药卫生—中药学]