稀土铽离子与去甲肾上腺素的荧光反应及其分析应用  

Fluorescence Reaction of Terbium(Ⅲ) Ion and Norepinephrine and Its Analytical Application

作　　者：童裳伦[1] 项光宏[1] 陈萍萍[1] 

机构地区：[1]浙江大学环境科学研究所,浙江杭州310028

出　　处：《光谱学与光谱分析》2004年第12期1612-1614,共3页Spectroscopy and Spectral Analysis

基　　金：浙江省自然科学基金 (2 0 0 0 84);浙江省分析测试基金资助项目

摘　　要：实验发现稀土铽离子能与去甲肾上腺素发生络合反应 ,并发射出铽离子的特征荧光。在对铽离子与去甲肾上腺素的络合发光反应进行了详细研究的基础上 ,提出了一种简便、快速、灵敏检测去甲肾上腺素的新方法。在激发波长为 30 0nm ,发射波长为 5 4 5nm处测定其荧光强度 ,去甲肾上腺素浓度在 0 0 1～ 5 0 μg·mL-1范围内与体系的荧光强度具有良好的线性关系 ,相关系数为 0 9990 ,检出限为 1 0ng·mL-1(S/N=3)。该法应用于去甲肾上腺素药物的测定 ,结果令人满意。Experiments indicated that terbium(Ⅲ) ion could form complex with norepinephrine, then emitted its characteristic fluorescence. In the present paper, the fluorescence reaction of terbium(Ⅲ) ion with norepinephrine was studied in detail. Thus a new method for the determination of norepinephrine was propsed. The fluorescence intensity was measured in a 1 cm quartz cell with the excitation and emission wavelengths of 300 and 545 nm, respectively. The result showed that the fluorescence intensity of the system presented a linear relationship with the concentration of norepinephrine in the range of 0.01-50 μg·mL -1, and the detection limit(S/N=3) was 1.0 ng·mL -1. The method was used to determine pharmaceutical sample which contained norepinephrine, and the result was satisfactory.

关 键 词：铽离子 去甲肾上腺素 荧光分析法 稀土元素 儿茶酚胺 酪氨酸 测定方法 

分 类 号：R446[医药卫生—诊断学] O657.3[医药卫生—临床医学]