野山人参和栽培人参的DALP指纹图谱  

Distinguishing Wild Panax ginseng from Cultivated Panax ginseng Based on Direct Amplification of Length Polymorphism(DALP) Analysis

作　　者：王琼[1] 程舟[1] 张陆[1] 万树文 丁建弥 傅大煦[1] 陈家宽[1] 张文驹[1] 

机构地区：[1]复旦大学生命科学学院生物多样性科学研究所生物多样性与生态工程教育部重点实验室,上海200433 [2]上海雷允上药业有限公司神象参茸分公司,上海200002

出　　处：《复旦学报（自然科学版）》2004年第6期1030-1034,共5页Journal of Fudan University：Natural Science

基　　金：上海市科技发展基金资助项目(01DJ19006;03DZ19547)

摘　　要：采用DALP分子标记技术寻找野山人参和栽培人参DNA之间的特异性差异.选取10个野山人参和5个栽培人参(包括一个移山参),通过改进微量人参DNA提取的方法和优化DALP PCR实验体系,得到了人参的DALP指纹图谱.图谱显示,野山人参的遗传多样性远高于栽培人参,野山人参与栽培人参图谱存在差异,且各自存在一条特异性条带.结果表明,DALP分子标记技术可以用来进行野山人参和栽培人参的遗传差异研究.To distinguish wild Panax ginseng from cultivated Panax ginseng, high quality DNA were extracted from ginseng material of 5 mg or so. Based on improved SDS method, genomic fingerprints of ten individuals of wild ginseng and five of cultivated ginseng were obtained using direct amplification of length polymorphism (DALP)analysis. The DALP fingerprints showed that the genetic variation in wild ginseng group was much higher than that in cultivated ginseng group. The characteristic DALP fragments, which could be used to distinguish wild ginseng from cultivated ginseng were found. DALP has proved to be an effective technique to study DNA difference between wild ginseng and cultivated ginseng.

关 键 词：人参 指纹图谱 特异性 移山参 PCR DNA 改进 栽培 分子标记技术 遗传差异 

分 类 号：R282.71[医药卫生—中药学] R284[医药卫生—中医学]