缺血(氧)鼠心肌肌质网和线粒体内钙离子变化的研究  

The Change of Ca^(2+) in SR and MIT of Isolated Rat Heart

作　　者：车成日[1] 崔禹锡 李香善[1] 金基焕[1] 王明勇[1] 李素香[1] 李丹[1] 

机构地区：[1]延边医学院附属医院胸心外科 [2]北京天坛医院胸外科

出　　处：《延边医学院学报》1993年第1期43-46,48,共5页Journal of Medical Science Yanbian University

摘　　要：用液体闪烁计数法测定离体再灌注鼠心肌肌质网(SR)和线粒体(Mit)内^(45)Ca^(2+)放射性强度(cpm),比较能量制剂ATP-氯化镁,活性氧自由基清除剂SOD和钙阻滞剂Verapamil对离体缺血(氧)再灌注鼠心肌细胞SR和Mit钙离子浓度的影响。结果表明,ATP-氯化镁,SOD和Verapamil组SR内^(45)Ca^(2+)的cpm均高于对照组cpm(P<0.01或P<0.05),而Mit内均低于对照组cpm(P<0.01)。此3种药物均能增高离体大鼠心肌细胞内SR^(45)Ca^(2+)和降低Mit^(45)Ca^(2+)积聚,从而保护心肌细胞缺血再灌注损伤。The strength of irradiation of ^(45)Ca^(2+) in sarcoplasmic reticulum vesicle (SR) and mitochondria (Mit) was tested by liquid scintillation counting method. Comparing the effections of ATP-MgCl_2, SOD and verapamil to the ^(45)Ca^(2+) concentration in SR and Mit which separated from isolated isolated ischemia rat hearts. The results show that the ^(45)Ca^(2+) cpm of three groups higher than the control group in SR and lower than the control group in Mit. That means all the three kinds of reagent can protect reperfusion injury of heart cell through enhance SR Ca^(2+) and inhibit the accumulation of Ca^(2+) in Mit.

关 键 词：肌质网 线粒体 钙 心肌缺血 再灌注 

分 类 号：R542.202[医药卫生—心血管疾病]