紫叶李组织培养及快繁体系的建立  

Tissue culture and rapid propagation of readleaf cherry plum in vitro

作　　者：孙在红[1] 夏阳[2] 梁慧敏[2] 刘荣堂[1] 

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070 [2]山东省林业科学研究院,山东兰州250014

出　　处：《草原与草坪》2005年第1期58-61,共4页Grassland and Turf

基　　金：山东省科技发展计划(011100104)资助项目;国家"863"(2001AA244082)

摘　　要：试验以紫叶李春季萌生苗嫩枝为外植体,在腋芽诱导的基础上,建立“以芽尖诱导丛生芽”的组培快繁体系。腋芽诱导培养基为MS+6BA0.5mg/L+NAA0.1mg/L与MS+6BA1.0mg/L+NAA0.1mg/L;丛生芽增殖培养基为MS+BA1.0mg/L+IBA0.3mg/L;壮苗培养基为MS+6BA0.4mg/L+IBA0.1mg/L+GA30.2mg/L。最佳生根培养基为1/2MS+IBA1.0mg/L+蔗糖20g/L。Tissue culture and rapid propagation system of Redleaf cherry plum was established by using sprouted branch of spring as explants, base on of axillary buds inducing.Medium foraxillary buds inducing wasMS+6-BA 0.5 mg/L+NAA 0.1 mg/L and MS+6-BA 1.0 mg/L+NAA 0.1mg/L,for adventitious buds proliferation was MS+BA 1.0 mg/L+IBA 0.3 mg/L, for strong seedling was MS +6-BA 0.4 mg/L+IBA 0.1 mg/L+GA (3) 0.2 mg/L.T he optimum medium for shoot inducing was 1/2 MS+IBA 1.0 mg/L+source 20 g/L.

关 键 词：紫叶李 组织培养 丛生芽增殖 植物激素 

分 类 号：S336[农业科学—作物遗传育种]