离子选择电极法测定孕妇血清中无机氟  

Determination of inorganic fluoride in serum of pregnant women by ISE

作　　者：景立新[1] 冯仲扬[1] 刘艳平[1] 景建瑜[1] 陆继辉[1] 周白灵[1] 姜英慧[1] 高卫[1] 迟庆[1] 

机构地区：[1]大连大学医学院,辽宁大连116622

出　　处：《大连大学学报》2004年第6期49-52,共4页Journal of Dalian University

摘　　要：[目的]观察妇女妊娠前后血清中氟化物变化.[方法]用离子选择电极,以标准曲线法、标准加入法检测孕妇血清中氟,并与未孕妇女血清进行了比对实验.[结果]本方法线性范围,氟离子浓度0.02-5.0mg／L,r=0.9999,6=59.7,(25℃),检出下限0.012mg／L,孕妇血清和当地未孕妇女血清氟含量分别为0.035±0.006mg/L,0.038±0.008mg／L,P<0.01,具有显著性差异.方法相对标准偏差在5.4％以下,回收率为96％-106％.[结论]本测定方法准确,灵敏,简便;妊娠妇女应注意孕期中的营养补充.[Objective]The fluoride change in serum of the pregnant women before and after the pregnancy was observed. [Methods]Using fluoride ion selective electrode method with standard curve and standard addition, to determine inorganic fluoride in serum of the pregnant women and compare with the women without gestation in the area. [ Results ] The linear range of the method was 0. 02-5. 0 mg/L, r = 0. 999 9, b =59.7, (25℃) , and the test lower limit was 0. 012 mg/L;serum fluoride in the two samples was 0. 035±0. 006 mg/L,0. 038±0. 008 mg/L, P < 0. 01. That showed obvious difference in two groups. The relative standard deviation was under 5.4%. The rate of recovery was 95% to 106%. [ Conclusions ] The method is accurate, sensitive and easy. Pregnant women should pay attention to nutritive addition during the pregnant period.

关 键 词：孕妇血清 无机氟 离子选择电极法 标准曲线法 

分 类 号：R714[医药卫生—妇产科学] R446.1[医药卫生—临床医学]