木素酶降解蔗渣浆残余木素机理的研究  

Study on Degradation of Residual Lignin in Bagasse Pulp by Ligninase

作　　者：莫佳琳[1] 王双飞[1] 宋海农[1] 黄显南[1] 

机构地区：[1]广西大学造纸科学研究所,广西南宁530004

出　　处：《中国造纸学报》2003年第2期20-23,共4页Transactions of China Pulp and Paper

基　　金：广西壮族自治区自然科学基金资助项目(0 2 36 0 0 3)

摘　　要：采用核磁共振氢谱 ( 1H NMR)和碳谱 ( 13 C NMR)的分析手段 ,研究白腐菌PhanerochaeteChrysosporium固体发酵分泌的木素酶降解蔗渣浆中残余木素的机理。1H NMR分析表明 ,经过木素酶预处理残余木素部分苯环结构发生了开环反应 ,产生CC双键结构 ,酚羟基含量降低 ,含酚羟基的木素优先降解 ,残余木素中的醇羟基含量升高 ;13 C NMR分析结果表明木素分子的Cα—Cβ连接键、部分侧链、β OThe mechanism of degradation of residual lignin in bagasse pulp during biobleaching with ligninase was studied by means of 1H-NMR and 13C-NMR. The 1H-NMR and 13C-NMR spectra showed that the structure of the residual lignin before and after the biobleaching were different. The treatment resulted in the break of phenyl which produced C=C. In addition, the content of phenolic hydroxyl was increased. This indicated that the lignin containing phenolic hydroxyl degraded firstly. 13C-NMR showed that the oxidative cleavage of Cα-Cβ bond, side chains, β-O-4 bond and methoxy group played an important role in delignification of the pulp.

关 键 词：残余木素 蔗渣浆 酚羟基 ^13C-NMR 酶预处理 固体发酵 氧化 酶降解 羟基含量 大分子 

分 类 号：TS79[轻工技术与工程—制浆造纸工程] TS77