血小板激活因子拮抗剂WEB2086对小鼠腹腔巨噬细胞释放肿瘤坏死因子的影响(英文)  

EFFECT OF PLATELET ACTIVATING FACTOR ANTAGONIST WEB 2086 ON THE PRODUCTION OF TNF FROM MURINE PERITONEAL MACROPHAGES

作　　者：鞠佃文[1] 郑钦岳[1] 王洪斌[1] 王晓峰 方军[1] 

机构地区：[1]第二军医大学药学院药理教研室,上海200433

出　　处：《药学学报》1993年第10期721-727,共7页Acta Pharmaceutica Sinica

摘　　要：小鼠ip3%TG 4天后取其腹腔巨噬细胞,血小板激活因子拮抗剂WEB 2086对LPS诱导的巨噬细胞释放肿瘤坏死因子(TNF)有显著的抑制作用。时效研究表明,TNF的产生和WEB 2086的抑制作用从LPS刺激后4 h开始,持续到22 h,在16 h时达到高峰。本文还首次采用一种用放线菌素D和NaF处理的L-929细胞测定TNF的新方法,此法与另外3种生物测定方法进行比较的结果显示此法具有更敏感、方便的特点。In the present study the effect of platelet activating factor (PAF) antagonist WEB 2086 on the production of tumor necrosis factor (TNF) from primed murine peritoneal macrophages was investigated. At 10_(-6) and 10_(-5) mol/L, WEB 2086 was found to significantly inhibit LPS induced TNF production from macrophages by activated thioglycollate solution. WEB 2086 inhibition of TNF release began 4 h after LPS stimulation and lasted 22 h with a peek at 16 h. The results showed that PAF might play an important role in the production of TNF. Four methods were compared in the bioassay of TNF. The data demonstrated that L-929 cells treated with actinomycin D and sodium fluoride were the most sensitive for the assay of TNF. This method was employed in this study.

关 键 词：肿瘤坏死因子 巨噬细胞 血小板激活因子 拮抗剂 

分 类 号：R965[医药卫生—药理学]