甲型肝炎早期实验诊断的研究  

INVESTIGATION OF EARLY LABORATORY DIAGNOSIS FOR HEPATITIS A

作　　者：尚守礼[1,2] 丛树梅 李莉[1,2] 陈虹[1,2] 徐皖苏 王耀宗[1,2] 

机构地区：[1]山东医科大学传染病学教研室 [2]济南市传染病医院

出　　处：《山东医科大学学报》1989年第4期31-33,共3页Acta Academiae Medicinae Shandong

摘　　要：以人胚肺二倍体细胞分离出的甲型肝炎病毒作抗原,以马抗人IgM经纯化后的抗体为捕捉抗体,以纯化甲型肝炎恢复血清为特异性抗体,过碘酸钠法标记酶,以OPD为底物制成抗-HAV IgM诊断试剂盒,建立了ELISA捕捉法检测抗-HAV IgM,结果:甲型肝炎暴发点84例均为阳性,流行性出血热及健康成人各30例均为阴性。322例急性黄疸型肝炎住院患者中192例阳性(占59.6%)。12例第一病日患者11例阳性(占91.6%),证明该方法对甲型肝炎早期诊断有重要价值。An anti-HAV IgM test kit was prepared by using HAV cul- tured indiploid cells from human fetal lung as antigen,commercial purified horse to human IgM as capture antibody,purified serum from patients with hepatitis A(HA)in convalescence as specific antibody enzyme-labeled with sodium periodicate and PPD as substrate.A method for determining the anti- HAV IgM with ELISA IgM antibody eapture assay was established.The test showed that the 84 patients with HA in epidemic area were positive,30 cases of health adults and with epidemic hemorrhagic fever were negative and 192 out of 322 in patients with acute jaundice hepatitis were positive (59.6%).11 out of 12 patients at first illnessday were positive(91.6%).

关 键 词：甲型肝炎 诊断 

分 类 号：R512.610.4[医药卫生—内科学]