用聚合酶链反应技术诊断结核病  

Use of Polymerase Chain Reaction for Rapid Diagnosis of Tuberculosis

作　　者：闵运兵[1] 卓礼梅[1] 姚俊霞[1] 綦玉兰[1] 

机构地区：[1]广州市东山区人民医院,中山医科大学微生物与免疫学教研室,中山医科大学病理学

出　　处：《同济医科大学郧阳医学院学报》1994年第2期96-98,共3页

摘　　要：用聚合酶链反应技术对结核杆菌特异性重复序列ＩＳ６１１０部分基因１２３ｂＰ的片段进行扩增。该法检测人型结核杆菌灵敏度可达到１０ｆｇＤＮＡ，对１４９份结核患者临床标本检测结果显示ＰＣＲ总阳性率（６９．１％）明显高于直接涂片荧光镜检（１５．４％）与细菌培养（１０．１％）的阳性率（Ｐ＜０．０１）。研究表明ＰＣＲ是一种特异、敏感、快速诊断结核病的好方法，值得推广。A 123bp segment derived from the repetitve DNA IS6110 specific for Mycobacteria tuberculosis was aplified by using poly merase chain reaction (PCR).The assay could detect as less as 10fg DNA of M.tuberculosis.It was used to patients with tuberculosis (67 sputa, 53 pleural fluid aspipositivity rate of PCR (69.1%) was signmificantly higher than that of conventional fluorescent staining(15.4%)and culture technique(10.1%)，Our results suggest that PCR may be a specific,sensitive and rapid technique for diagnosis of tuberculosis.

关 键 词：聚合酶链反应技术 诊断 结核病 结核杆菌 

分 类 号：R52[医药卫生—内科学]