血清HBV-DNA检测的临床价值  

Clinical Uses of Detection of Serum Hepatitis B Virus DNA Using Dot-hybridization and Polymerase Chain Reaction

作　　者：陈永良[1] 张仲华[1] 黄永章[1] 陈刚[1] 

机构地区：[1]郧阳医学院医学实验中心,上海医科大学免疫学教研室

出　　处：《同济医科大学郧阳医学院学报》1994年第3期141-143,共3页

摘　　要：选用分子斑点杂交法和多聚酶链反应（ＰＣＲ）观察１０７例疑为乙型肝炎患者ＨＢＶ－ＤＮＡ检出情况，分析ＨＢＶ－ＤＮＡ与ＨＢＶ血清抗原抗体标记物检出的关系，初步比较目前采用的这两种方去，认为分子斑点杂交法在应用中优于ＰＣＲ，值得推广。Polymerase chain reaction(PCR) assay and dot-hybridization test were used in sera of 107 patients to detect hepatitis B virus(HBV) DNA. The results of two methods were similer. Detection of HBV-DNA in serum was obviously relative to patients with positive for HBeAg or HBeAb, if HBsAg and HBcAb was positive in dot-hybridization test; 3 patients were positive for HBV-DNA in 4 cases who were negative for HBsAg,HBeAg,HBeAb and HBcAb.These data showed that dot-hyridization test was useful in detection of serum HBV-DNA.

关 键 词：血清HBV-DNA 检测 多聚酶链反应 分子斑点杂交法 乙型肝炎 

分 类 号：R512.62[医药卫生—内科学] R446.6[医药卫生—临床医学]