枸杞多糖对高温损伤小鼠睾丸曲细精管影响的电镜观察  

EFFECT OF LYCIUM BARBARUM ONMICE'SPERMATOGENIC CELL INJURED BY HEAT

作　　者：胡庆和[1] 韩斌[1] 张焱[1] 赵承军[1] 阎廷雄[2] 

机构地区：[1]宁夏医学院中心实验室 [2]宁夏医学院附属医院泌尿外科

出　　处：《宁夏医学院学报》1994年第2期112-115,共4页Journal of Ningxia Medical College

摘　　要：为研究枸杞多糖对小鼠睾丸生精细胞的作用，本次以热吹风器对小鼠双侧睾丸透热（Ｂ组），另取正常不透热小鼠为空白对照（Ａ组），蒸馏水灌胃对照（Ｃ组）和透热后灌服枸杞多糖（Ｄ组），进行实验观察，待Ｂ、Ｃ、Ｄ各组于透热结束后即刻、３天、１周、２周、５周处死动物，取双侧睾丸，常规作光镜和电镜观察。实验结果表明，Ｂ组各期生精细胞进行性受损，属肿胀溶解性细胞变性坏死过程，Ｄ组则在１周时受损部位和程度未见进行性加重，５周时基本恢复正常，提示枸杞多糖有明显的抗高温、保护生精细胞作用。This study was carried out for investigating the effects of LyciumBarbarum(L,B.)on mice spermatogenic cells injured by heat.32 micewere devided randomly and equally into four groups.Each testis of groupB mice was diathermized with a heat blower.Group A consists of eighthealthy mice without any treatment, which were used as a blank control.Mice of another contro1,group C,were treated by intragastric perfusionwith distilled water.Mice of group D were treated by testicular dia-thermy and intragastric perfusion with polysaccharide of L.B.Aftertreatments,the mice of group B,C,D were killed immediately, threedays,one week,two weeks,and five weeks later separately,All thetestes of the killed mice were examined by microscopy and electronmi-croscopy.The results showed that there were progressively necrotizingprocesses of spermatogenic cells in group B,including cloudy swellingand cytolysing,But in group D, there was no progressive aggravation ofthe pathological clianges in the one week specimen.And the changesreversed to normal gradually in about five weeks, indicating that poly-saccharide of L.B.is of significant antihyperthermic and protectiveeffect on spermatogenic cells.

关 键 词：枸杞多糖 小鼠 睾丸曲细精管 双侧 进行性 正常 生精细胞 变性 电镜观察 动物 

分 类 号：R285.5[医药卫生—中药学] R697.2[医药卫生—中医学]