子宫内膜异位症不孕患者腹腔液对小鼠2-细胞期胚胎发育的影响  

In vitro Study on the Effect of Peritoneal Fluid of Infertile Patients with Endometriosis on the Development of Two-cell Mouse Embryos

作　　者：刘义[1] 罗丽兰[2] 赵海波[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,武汉430022 [2]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《华中科技大学学报（医学版）》2003年第4期419-420,424,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省自然科学基金资助 (No .95J4 3)

摘　　要：目的　观察子宫内膜异位症不孕患者腹腔液对早期胚胎发育的影响。方法　将 12例轻度子宫内膜异位症不孕和 10例非子宫内膜异位症不孕患者腹腔液作用于小鼠 2 细胞期胚胎 ,每隔 12h观察 1次 ,记录 4 8h后期 细胞、8 细胞和 16 细胞阶段的胚胎数。结果　胚胎体外培养 4 8h后 ,两组 4 细胞、 8 细胞和 16 细胞阶段胚胎发育率分别为30 7%、 14 0 %、 32 5 %和 14 3%、 2 9 8%、 4 7 6 % ,其差异有极显著意义 ,P <0 0 1。结论　子宫内膜异位症患者腹腔液对早期胚胎发育有毒性作用 ,是导致患者不孕的原因之一。Objective To observe the effect of peritoneal fluid of infertile patients with endometriosis on the development of early embryos. Methods Peritoneal fluids from 12 infertile women with endometriosis (EMT group) and 10 infertile women with normal pelvic (control group) were applied to act on the two cell mouse embryos. The 4 cell, 8 cell and 16 cell mouse embryos were recorded within 48 h. Results After the two cell mouse embryos were cultured for 48 h, the percentage of 4 cell, 8 cell and 16 cell stage in EMT group was 30 7 %, 14 0 %, 32 5 % respectively versus 14 3 %, 29 8 %, 47 6 % in control group with the difference being very significant ( P <0 01). Conclusion The embryo toxicity of the peritoneal fluid may be involved in the pathogenesis of infertility associated with endometriosis.

关 键 词：子宫内膜异位症 不孕症 腹腔液 胚胎毒性 

分 类 号：R711.71[医药卫生—妇产科学]