黄芪水提物和知母甙元ZMS对28～30月龄大鼠外周血淋巴细胞受体的调节作用  

The Regulation effect of Huangqi decoction and sapogenin ZMS from Zhinuon peripheral β-adrenoceptors in 28-30 month old rats

作　　者：孙启祥[1] 金立玲[1] 易宁育[1] 

机构地区：[1]上海第二医科大学细胞调控研究室

出　　处：《中药药理与临床》1994年第5期12-15,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：以１２５Ⅰ－心得静为放射配基，测定完整淋巴细胞β受体。多点法可得到典型单位点饱和曲线。在饱和区选定配基浓建立单位法，测定ＳＤ大鼠外周血淋巴细胞β受体的密度。结果２８～３０月龄鼠明显低于５～６月龄成年鼠。长期口服补气药黄芪有明显上调作用，而长期口服知母甙元ＺＭＳ无类似作用。以上结果与老龄时脑β受体降低及黄芪有上调作用而知母生地无上调作用基本吻合。technique for measuring β-adrenoceptor density of intact lymphocpe with 125I-Pindolol was established. Typicalsingle site saturation curve was obtained with multi-point method. A suitable 125I-pindolol concentration was selectedfrom the saturation curve for the single-point method and with this method, the β-adrenoceptor densities of the peripheral lymphocytes of old (28-30 montha old) and adult (5-6 months old) SD rats were determined. It was found thatthe rasptor density was significantly lower in the old group than that in the young group (1l. 45±0. 15 versus 1. 86±0.17 fmol/106cells. P<O. 01). Which is in agreement with the change of β-adrenoceptors in btain. and that whentestal drugs orany given to the old SD rats. Huangqi (Astragnlus membranaceus Bge. ) could significantly resutate upthe β-adrenoceptor density (2. 23±0. 31 versue 1. 49±0. 19 f mol/10 calls, P<0. 01) but sapogenin ZMS fromZhimu (Anemarrhena asphodeloides Bge. ) could not do so. which is also similiar to the response of brain β-adrenoceptors to the two drugs.

关 键 词：外周血淋巴细胞 Β受体 知母 长期口服 黄芪水提物 大鼠 调节作用 降低 配基 密度 

分 类 号：R285.5[医药卫生—中药学] R562.2[医药卫生—中医学]