浙江省2003—2004年甲副伤寒菌株质粒图谱分析  

Plasmid profile pattern of S.paratyphi A strains isolated in Zhejiang province during 2003～2004

作　　者：褚佩英[1] 朱水荣[1] 陈恩富[1] 张政[1] 叶菊连[1] 莫顺堂[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310009

出　　处：《中国卫生检验杂志》2005年第2期138-139,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:探讨浙江省2003、2004年不同地区分离的甲型副伤寒菌株质粒基因定位及与致病性的关系。方法:利用技术及电泳图谱方法,对浙江省2003、2004年暴发点病人和住院病人分离的部分甲型副伤寒菌株进行质粒图谱分析并做药敏试验。结果:分析发现选择的78株菌株中, 78株( 100% )对红霉素(ETM)耐药, 77株(98. 72% )对利福平(RFP)、链霉素(SM)及头孢拉定(CV)耐药, 76株(97 .44% )对羧苄青霉素(CAR)及磺胺(SD)耐药,64株(82 .05% )对强力霉素(DC)耐药;大多数菌株带有一20kb大小质粒,所有菌株均带有2 2kb大小的质粒。结论:甲型副伤寒菌对ETM、RFP、CAR、CV、SM、SD、DC耐药,并认为具有多重耐药性是浙江省甲型副伤寒发病率持续高发的主要原因。Objective:To analysis the relationship between the bacterium pathologinesis and the plasmid genetic characterization in Zhejiang province during 2003～2004. Methods: 78 isolates of S.paratyphi A from the patients in the broken-out spots and inpatients were analysed by plasmid profile, the resistance was also studied. Results: Among 78 isolates of S.paratyphi A analysed, 78(100%) isolates were resistant to Erythromycin; 77 isolates(98.72%) to Rifampicin and to Ceflazidime as well as to Streptomycin; 76 isolates (97.44%) to Carboxy Benzylpenicinllin and to Sulfadiazine, SD.; 64 isolates (82.05%) to Doxycycline. Most of the isolates harbour one 20kb plasmid, and all of the isolates harbour one 2.2 kb plasmid. Conclusion: The finding indicate that S.paratyphi A were resistant to ETM、RFP、CAR、CV、SM、SD、DC and harbour one 20 kb plasmid and 2.2 kb plasmid;The multi-resistance of the isolates might continually serve the major reasons of the higher morbidity of typhoid fever in Zhejiang province recently.

关 键 词：耐药 甲型副伤寒菌 质粒图谱分析 菌株 RFP SD DC 羧苄青霉素 电泳图谱 基因定位 

分 类 号：R378[医药卫生—病原生物学] R446.5[医药卫生—基础医学]