乳香对几种病原微生物抗性作用的初步研究  

Primary study on resist several pathogenic microbe of frankincense

作　　者：饶本强[1] 李福荣[1] 张海宾[1] 

机构地区：[1]信阳师范学院生命科学学院,河南信阳464000

出　　处：《信阳师范学院学报（自然科学版）》2005年第1期54-56,共3页Journal of Xinyang Normal University(Natural Science Edition)

摘　　要：研究了树脂中药乳香对几种常见致病菌的抗性作用 .在无菌条件下制作乳香悬浊液并稀释成不同梯度浓度 ,通过药物敏感性试验滤纸片扩散法和最低抑菌浓度测定 ( MIC)分别对大肠埃希氏杆菌( Escherichia coli)、金黄色葡萄球菌 ( Staphylococcus aureus)、蜡状芽孢杆菌 ( Bacillus cereus)、铜绿假单胞菌( Pseudomonasaeruginosa)、普通变形杆菌 ( Proteusvulgaris)进行测定 .结果表明平均抑菌圈直径由大到小依次为 :大肠埃希氏杆菌 1 1 .5 3 mm、普通变形杆菌 1 1 .2 8mm、铜绿假单胞菌 1 0 .2 4mm、金黄色葡萄球菌 9.44mm,蜡状芽孢杆菌 8.73 mm;测定这 5种致病菌的最低抑菌浓度 ( MIC)得到 :大肠杆菌为 1 /2 5 6g· m L- 1、普通变形杆菌为 1 /2 5 6g· m L- 1 、铜绿假单胞菌为 1 /1 2 8g· m L- 1、金黄色葡萄球菌为 1 /64 g· m L- 1 、蜡状芽孢杆菌为 1 /1 6g· m L- 1 .研究表明乳香对大肠杆菌的抗性最强 ,对蜡状芽孢杆菌的抗性最弱 ,采用滤纸片扩散法和最低抑菌浓度测定 ( MIC)得到的结果基本一致 .研究还表明乳香具有广谱抗菌作用 。This paper discusses the effect of frankincense resisting several pathogenic microbe. Makes frankincense's latex under asepsis condition, and then dissolves frankincense into water to confect different density of gradient. By adopting medication susceptivity experiment, the filter paper diffusion and the minimum inhibition concentration mensuration(MIC), respectively to detect E. coli, S. aureus, B. cereus, P.aeruginosa and P.vulgaris. According to average inhibition diameter from big to small, the results are as follows: E.coli 11.53 mm, P.vulgaris 11.28 mm, P.aeruginosa 10.24 mm, S.aureus 9.44 mm, B.cereus 8.73 mm; Mensurating MIC of this five pathogenic microbes to set out: E.coli 1/256 g·mL^(-1), P.vulgaris 1/256 g·mL^(-1), P.aeruginosa 1/128 g·mL^(-1), S.aureus 1/64 g·mL^(-1), B.cereus 1/16 g/mL^(-1). The results indicate that among these all species, the restraint effect of E. coli is the most obvious, that of B.cereus is the most feeble. By and large, this two experimental results are nearly same.Additionaly,The results also indicate that there is wide table bacteriostasis in frankincense, but the inhibition power to different bacteria is different.

关 键 词：乳香 抗性作用 滤纸片法 最低抑菌浓度测定(MIC) 

分 类 号：Q935[生物学—微生物学]