人卵和胚胎线粒体ATP合酶表达的研究  

ATP Synthase Expression in Human Unfertilized Oocytes and Cleavage-stage Embryos

作　　者：黄睿[1] 彭文林[2] 张敏芳[2] 庄广伦[2] 

机构地区：[1]广东省妇幼保健院集爱遗传与不育诊疗中心,广州510010 [2]中山大学附属第一医院生殖中心,广州510089

出　　处：《华中科技大学学报（医学版）》2005年第1期92-93,100,F004,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：广东省自然科学基金资助项目 (No 04300799)

摘　　要：　目的　探讨ATP合酶表达与卵子发育潜能间的关系。方法　收集行常规体外受精 (IVF) 和单精子卵胞质内注射 (ICSI) 不受精的成熟卵母细胞25个, 不适合移植及冷冻保存的胚胎30 个, 应用免疫组织化学方法检测单个卵子和胚胎ATP合酶的蛋白表达。结果　ATP合酶均匀地表达于人卵和胚胎的卵裂球中。未受精人卵中表达呈弱阳性; 胚胎中的表达呈强阳性; 有碎片的胚胎中, 碎片部位ATP合酶的表达明显较卵裂球降低。结论　ATP合酶蛋白表达减少可能通过干扰卵子的能量代谢以及正常的受精过程而参与受精失败, 并与卵子的发育潜能密切相关。Objective To explore the relationship between the expression of ATP synthase and oocyte developmental potential.Methods Twenty-five unfertilized oocytes and 30 embryos unsuitable to transfer and cyropreservation were collected.Immunocytochemistry was performed to detect the expression of ATP synthase.Results ATP synthase was expressed in both oocyte and embryo.The immunoreactivity was weak in oocyte cytoplasm, while it was strong in embryo cytoplasm.In embryo with fragmentation, the immunostaining within intact blastomere was stronger than the staining within fragmentation.Conclusion The decreased expression of ATP synthase might involve in fertilization failure through interfering energy metabolism and fertilization process, and the ATP synthase's level is relevant to the developmental potential of oocytes.

关 键 词：人卵 胚胎 酶表达 卵子 线粒体 免疫组织化学方法 ICSI ATP合酶 卵裂球 发育 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学] Q945.11[医药卫生—基础医学]