贵州苗族新生儿HbF中G_λ/A_λ比值测定与~Aγ~T基因频率分析  

Ratios of ~Gγ and ~Aγ Chains,and ~Aγ~T Gene Frequency in Miao Nationality Newborns in Guizhou Province

作　　者：易志勤[1] 南国华[2] 陈松森[3] 梁植权[3] 姚源黎 杨文鸿 王立荣 丁桂华 

机构地区：[1]江西省赣南医学院生物化学教研室,赣州341000 [2]江西医学院生物化学教研室,南昌330006 [3]中国医学科学院基础医学研究所,北京100005 [4]贵州省凯里市妇幼保健院,凯里556000

出　　处：《遗传》1993年第3期1-3,共3页Hereditas（Beijing)

摘　　要：用含Triton X-100的酸性尿素聚丙烯酰胺凝胶电泳(PAGE)法测定了贵州省108例苗族新生儿脐带血胎儿血红蛋白(HbF)中。~Gγ/~Aγ值。显示107例(占99.07％)新生儿的~Gγ值在55—78％之间,均值与标准差为70.42±3.45;高~Gγ值者1例(占0.925％);低~Gγ值者未发现。用Large-pore Vydac C4柱反相高效液相层析法对108例样本进行了~Aγ~T基因频率(f^4γ~T)分析,f^4γ~T为0.0648。The ratio of Gγ to Aγ of fetal hemoglobin in 108 cord blood samples of Miao nationality newborns in Guizhou province was determined by the polyacrylamide gel electrophoresis(PAGE).The result showed that one case had high Gγr value(0.925%) and 107 babies the normal Gγ values of 55 to 78%(70.42+3.45).The AγT gene frequency(fAγT) analysis in 108 samples was done by high pressure liquid chromatography using a large pore Vydac C4 column,the fAγT was 0.0648.

关 键 词：血红蛋白F 苗族 基因频率 

分 类 号：Q987[生物学—遗传学]