Pterocarine, a New Diarylheptanoid from Pterocarya tonkinesis, its Cell Cycle Inhibition at G_0/G_1 Phase and Induction of Apoptosis in HCT-15 and K562 Cells  

作　　者：HongBingLIU ChengBinCUI BingCAI QianQunGU DongYunZHANG QingChunZHAO HuaShiGUAN 

机构地区：[1]TianjinInstituteforBiomedicinalResearch（TIBiR）,Tanjin300384 [2]KeyLaboratoryofMarineDrugs,MinistryofEducation,China MarineDrugandFoodInstitute,OceanUniversityofChina,Qingdao266003／／BeijingInstituteofPharmacologyandToxicology,AMMS,Beijing100850 [3]KeyLaboratoryofMarineDrugs,MinistryofEducation,China MarineDrugandFoodInstitute,OceanUniversityofChina,Qingdao266003

出　　处：《Chinese Chemical Letters》2005年第2期215-218,共4页中国化学快报（英文版）

基　　金：This work was supported by the Fund from the National Natural Science Foundation of China(C.-B.CUI,No.39825126);the Fund for the 973-project from Ministry of Science and Technology(C.-B.CUI,No.1998051113),China;the Fund for Cheung Kong Scholar(C.B.CUI)from Cheung Kong Scholars Program,Ministry of Education of China.

摘　　要：Pterocarine (1), a new diarylheptanoidal compound, was isolated from Pterocaryatonkinesis (Franch.) Dode. together with a known diarylheptanoid, myricatomentogenin (2), througha bioassay-guided fractionation procedure. The structure of 1 was elucidated as (+)-3', 4''-epoxy-1-(4'-hydroxyphenyl)-7-(3''-hydroxyphenyl)-heptane-3-one by the spectroscopic methods.Pterocarine (1) inhibited the proliferation of tsFT210, HCT-15 and K562 cells with the inhibitionrates of 20.2±2.4, 23.8±2.4 and 50.5±1.2% at 100 μg/mL, respectively. Flow cytometric analysisindicated that 1 could inhibit the cell cycle of tsFT210, HCT-15 and K562 cells at the G0/G1 phaseand could also induce apoptosis in HCT-15 (19%) and K562 (11%) cells.Pterocarine (1), a new diarylheptanoidal compound, was isolated from Pterocaryatonkinesis (Franch.) Dode. together with a known diarylheptanoid, myricatomentogenin (2), througha bioassay-guided fractionation procedure. The structure of 1 was elucidated as (+)-3', 4''-epoxy-1-(4'-hydroxyphenyl)-7-(3''-hydroxyphenyl)-heptane-3-one by the spectroscopic methods.Pterocarine (1) inhibited the proliferation of tsFT210, HCT-15 and K562 cells with the inhibitionrates of 20.2±2.4, 23.8±2.4 and 50.5±1.2% at 100 μg/mL, respectively. Flow cytometric analysisindicated that 1 could inhibit the cell cycle of tsFT210, HCT-15 and K562 cells at the G0/G1 phaseand could also induce apoptosis in HCT-15 (19%) and K562 (11%) cells.

关 键 词：Pterocarine DIARYLHEPTANOID phenolic compound structure cell cycle inhibitor apop-tosis inducer Pterocarya tonkinesis Juglandaceae. 

分 类 号：R282.71[医药卫生—中药学] R284[医药卫生—中医学]