硒对体外培养大鼠胚胎的发育毒性实验研究  

Study on Developmental Toxicity of Sodium Selenite in Rat Embryos in Virto

作　　者：李勇[1] 孙棉龄[1] 吴德生[1] 李质胜[1] 

机构地区：[1]华西医科大学公共卫生学院

出　　处：《四川环境》1994年第4期24-27,共4页Sichuan Environment

摘　　要：本实验用全胚胎培养方法探讨了硒对体外培养大鼠胚胎的发育毒性。结果表明硒对胚胎的损害有明显的剂量－反应关系．硒浓度为２．０ｕｇ／ｍｌ，首先受累及的器官是视觉系统，提示该系统是硒的主要胚胎毒性作用部位。高浓度时，硒对反映胚抬生长发育和器官形态学分比的各指标均有抑制作用。６．０ｕｇ／ｍｌ剂量组胚胎畸形率为６６．７％，主要表现是无眼泡、耳泡，心包积液，肢芽小．神经管未闭和体节异常．１２．０ｕｇ／ｍｌ．死胎率高过８８．９％。Based on the 48h culture3 of day 9.5 whole rat emtryos in serun, it has been proved that selenium would have embryotoxi-city in a dose related manner. maner.The forst selective uptake site was optic system in emtryos cultured in vitro,this result was of importance.All indexes correlated with embryonic growth and morphological differentations were inhibited at inhibited at high concentration. The incidence ofabnormal embryos was 66.7% at 6.0ug/ml. The abnormal embryos were characterized by failures of development of sen sensory placodes and limb buds, hydropericardium. open neural tube and somite abnormalities. The embryonic mortality was up to 88.9% in embryos with one to three somites exposed to 12.0ug/ml seleniun.

关 键 词：大鼠胚胎 发育毒性 体外培养 实验研究 剂量 作用部位 胚胎毒性 死胎率 器官 腋芽 

分 类 号：X503[环境科学与工程—环境工程] R995[医药卫生—毒理学]