表面活性剂对转枯草芽孢杆菌纤溶酶基因烟草根系BSFE分泌表达的调节  

Surface Active Agent's Regulating of Statement and Secretion of Bacillus subtilis fibrinolytic enzymic gene in Transgenic Tobacco's Root System

作　　者：王瑞刚[1,2,3] 王彦[4] 曹前进[5] 吴自荣[5] 王水平[5] 

机构地区：[1]内蒙古农业大学生物工程学院 [2]内蒙古农业大学甜菜生理研究所,呼和浩特010018 [3]华东师范大学生命科学学院,上海200062 [4]内蒙古农业大学甜菜生理研究所 [5]华东师范大学生命科学学院

出　　处：《中国农学通报》2005年第2期17-23,共7页Chinese Agricultural Science Bulletin

基　　金：上海市科委98重大项目"安舒克栓酶的药效及工艺研究"(项目编号:985419000-3)。

摘　　要：0.05%～8%(w/v)浓度的常用表面活性剂Triton×100、SDS、DMSO和Tween20可使转枯草芽孢杆菌纤溶酶(Bacillus subtilis fibrinolytic enzyme,BSFE)基因烟草增加BSFE在根系中的分泌或增加其在水培液中的稳定性,从而促进转BSFE基因烟草水培液BSFE活性的增加.其单位根体积下水培液BSFE活性与培养天数呈抛物线型变化趋势,可模拟为二次回归模型.这种促进作用与表面活性剂的浓度有关.一定浓度的Triton×100、SDS、DMSO和Tween20可作为转BSFE基因烟草根系分泌表达的有效化学调节剂.The activity of the trans-BSFE gene tobacco of cultural solution can be promoted by 0.05%~8%(w/v) often-used surface active agents such as Triton ×-100, SDS, DMSO and Tween20 via strengthening the ability of secretion of root system or the stability of BSFE in cultural solution. The activity of BSFE in cultural solution with its unit volume of root changed parabolicly with the days when it was cultured. It may be intimated like the quadratric regression model. The function of promotion related with the concentration of the surface-active agent. The fixed concentration of triton ×100, SDS, DMSO and Tween20 can be used as the effective chemical regulator of the trans-BSFE gene tobacco root systems statement and secretion.

关 键 词：烟草根系 水培 浓度 二次回归 根体积 烟草水 化学调节 纤溶酶 基因 SDS 

分 类 号：S572[农业科学—烟草工业] S436[农业科学—作物学]