PCR快速检测甲型副伤寒沙门菌鞭毛抗原H-a研究  

A Study on the Rapid Detection of Flagellum Antigen H-a of S. Paratyphi A by PCR

作　　者：朱水荣[1] 陈恩富[1] 张政[1] 楮佩英[1] 孟真[1] 叶菊连[1] 罗芸[1] 

机构地区：[1]浙江省疾病预防控制中心,浙江杭州310009

出　　处：《浙江预防医学》2005年第3期4-5,8,共3页Zhejiang Journal of Preventive Medicine

摘　　要：目的　研究快速、特异、灵敏的检测甲型副伤寒沙门菌鞭毛特异相H a的PCR法。方法　选择甲型副伤寒沙门菌鞭毛抗原H a基因的特异引物进行PCR扩增 ,检测甲型副伤寒沙门菌标准菌株和我省不同地区上送的 5 7株甲型副伤寒沙门菌以及非甲型副伤寒沙门菌的其它菌株 ;将甲型副伤寒沙门菌标准菌株按10 - 1 ～ 10 - 9稀释后扩增比较PCR的检测灵敏度。结果　所有甲型副伤寒沙门菌均在 3 2 9bp处出现甲型副伤寒沙门菌鞭毛抗原基因产物 ,非甲型副伤寒沙门菌株PCR结果均为阴性 ;PCR检测下限为 10 3cfu/ml。结论　PCR比传统细菌检测方法更特异、快速、灵敏、简便 ,为临床诊断、实验室筛查以及流行病学快速查源、溯源提供了新的手段。Objective To establish a PCR assay for detection of Flagellum Antigen H a of S. paratyphi A.Methods The specific primers of flagellum antigen H a of S. paratyphi A were selected to be amplified by PCR assay to detect the S. paratyphi A of standard kind and 57 kinds of S. paratyphi A and other kind of non S. paratyphi A from different area in Zhejiang province. The sensitivity of PCR assay was analyzed with 10 -1 ～10 -9 diluted. Results The gene product of flagellum antigen H a was detected in all S. paratyphi A at 329bp, but was not detected in non S. paratyphi A. The lower limit of PCR detection was 103cfu/ml. Conclusions PCR assay is better than traditional detection for its specificity, celerity,sensitivity and convenience and it is helpful in clinical diagnosis, laboratory screen and epidemiological investigation.

关 键 词：甲型副伤寒沙门菌 聚合酶链反应(PCR) H-a基因 快速检测 

分 类 号：R516.3[医药卫生—内科学] R446.1[医药卫生—临床医学]