脑血管周围组织通道的功能(英文)  

Functions of cerebral perivascular tissue channel

作　　者：王俊华[1] 田牛[2] 郑世荣[2] 刘育英[2] 刘凤英[2] 李玉珍[2] 刘秀华[2] 赵秀梅[2] 韩岳[2] 蔡莉蓉[2] 

机构地区：[1]解放军空军总医院心内科,北京市100036 [2]解放军总医院病理生理研究室,北京市100853

出　　处：《中国临床康复》2005年第1期218-219,F003,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:在缺乏淋巴管的脑组织内,脑组织液与脑脊液间、脑组织液与淋巴系统间以及各部位脑实质之间的物质传递的过程与途径是一个被忽略的未知领域,这就是本文要探讨的脑组织通道,对指导中枢神经系统疾病的预后与康复干预具有重要意义。目的:探讨脑血管周围组织通道的功能,为研究脑组织的病理生理机制提供理论依据。设计:完全随机对照实验研究。单位:解放军空军总医院心内科;解放军总医院病理生理研究室。材料:实验在解放军总医院病理生理研究室完成。选择雄性SD大鼠20只,体质量250～300g,购自解放军总医院动物中心。干预:将大相对分子质量的异硫氰酸荧光素标记的白蛋白(fluoresceinisothiocyanatelabelledalbumin,FA及相对分子质量较小的异硫氰酸荧光)素标记的右旋糖酐(fluoresceinisothiocyanatelabelleddextran,FD)分别定位注射入大鼠脑内,一定时间后通过示踪剂与血管同时显影的方法观察注入的荧光物质在脑内的分布情况及其与组织结构间的关系。主要观察指标:荧光显微镜下观察注入的荧光示踪剂在脑内的分布情况、与血管之间的关系;对比分析注射不同相对分子质量荧光示踪剂实验组之间示踪剂分布的差异。结果:注入FA,FD之后均可见微血管周围广泛分布荧光浓集细胞(Mato细胞);FD在脑内扩散速度大于FA;BACKGROUND:The substance transmitting process and pathway in cerebral tissues in which there are lack of lymphatic vessels,between cerebral tissue fluid and c erebral spinal fluid,between cerebral tissue fluid and lymphatic system,as well as among cerebral substances of every region are the neglected unknown field and the cerebral tissue channel probed in the article,which will provide significan t importance in guiding prognosis and rehabilitation interventions of the disord ers of central neural system(CNS). OBJECTIVE:To probe into the functions of cerebral perivascular tissue channel so as to provide the theoretical evidences for studying the mechanism on patholo gy and physiology of cerebral tissues. DESIGN:Completely randomized and controlled experimental study. SETTING:Department of Cardiology,General Hospital of Air Force of Chinese PLA, and Department of Pathophysiology, General Hospital of Chinese PLA. MATERIALS:The experiment was performed in the Department of Pathophysiology,Ge neral Hospital of Chinese PLA.Twenty male SD rats, body mass varied from 250 g t o 300 g were purchased from Animal Center of Chinese PLA General Hospital. INTERVENTIONS:Fluorescein isothiocyanate labeled albumin(FA) with relative lar ge molecular mass and fluorescein isothiocyanate labeled dextran(FD) with relati ve small molecular mass were injected in localization in the brains of rats resp ectively.A certain time later,both the distribution of fluorescein substance in the brain and its relation with tissue structure were observed with both tracer agents and vascular developing method simultaneously. MAIN OUTCOME MEASURES:To observe the distribution of injected tracer agents in the brain and its relation with vessels under fluorescein microscope,to compare and analyze the differences in the distribution of tracer agents of different r elative molecular masses among the experimental groups. RESULTS:Fluorescein concentrated cell(Mato cell) presented extensively around micro vessels after injection of both FA a

关 键 词：脑/病理学 淋巴系统 显微镜检查 

分 类 号：R74[医药卫生—神经病学与精神病学]