铵盐存在下铝对辅酶NADH的结构的影响  

Study on the Effect of Aluminum (Ⅲ) on NADH in the Presence of NH_4Ac in Aqueous Solution

作　　者：杨小弟[1,2] 缪强[1] 钱胜利[1] 毕树平[1] 

机构地区：[1]南京大学配位化学国家重点实验室,化学化工学院

出　　处：《光谱学与光谱分析》2005年第1期79-82,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(20075011);教育部重点高校优秀青年教师基金资助项目

摘　　要：通过紫外光谱和核磁共振谱研究了溶液中铝与辅酶NADH相互作用。发现了与TrisHCl缓冲底液不同,在铵盐NH4Ac缓冲底液中,铝(Ⅲ)可以破坏辅酶NADH的二氢尼克酰胺环的结构,从而表现出对生物体中的转氨基作用的酶促反应的影响。This paper studied the interactions of Al (Ⅲ) and dihydronicotinamide adenine dinucleotide (NADH) in nearly neutral aqueous solutions (pH 6.5) by means of UV-Vis and ~ 1H, ~ ~13 C-NMR techniques. The results suggested that Al (Ⅲ) interacts with NADH to form Al-NADH complexes by occupying the binding sites of phosphate oxygen atoms O1_N and O1_A and ribose ring hydroxyl groups, which are the potential recognition sites for substrates, coenzyme and enzyme. In the presence of NH_4Ac salt buffer and with Al (Ⅲ) salt solution, NADH will be marked with structural changes at the nicotinamide moiety in contrast with almost no structural changes in Tris-HCl buffer solution with Al (Ⅲ) salt.

关 键 词：NADH 辅酶 影响 HC 发现 作用 紫外光谱 酰胺 氨基 核磁共振谱 

分 类 号：O614.812[理学—无机化学] R361[理学—化学]