四工作曲线法同时测定芦荟甙和芦荟大黄素  

Simultaneous determination of aloin and aloe-emodin by using four working curves

作　　者：庚梅[1] 杜海燕[1] 孙家跃[1] 吴爱萍[1] 

机构地区：[1]北京工商大学化学与环境工程学院,北京100037

出　　处：《分析试验室》2005年第1期66-69,共4页Chinese Journal of Analysis Laboratory

基　　金：北京市自然科学基金(2032007);北京市教委科技发展计划项目(KM200310011039)资助课题

摘　　要：利用芦荟甙和芦荟大黄素吸光度的良好加和性,提出了一种同时测定芦荟甙和芦荟大黄素的方法。在只加入氨水的情况下,分别作芦荟甙和芦荟大黄素的工作曲线;在加入氨水和高碘酸钠溶液的情况下,作芦荟甙和芦荟大黄素的另外两条工作曲线。样品在同样的两条件下,分别测出吸光度,由四条工作曲线联立方程组求解,即可同时求出芦荟甙和芦荟大黄素的量。芦荟甙的测定下限为0.0024mg/mL;芦荟大黄素的测定下限为0.0006mg/mL。样品分析结果相对标准偏差小于7%,加标回收率99%～101%。Based on the sum of absorbance, we proposed a new method for simultaneous determination of aloin and aloe-emodin. In the presence of ammonia, two working curves of aloin and aloe-emodin were made; In the presence of ammonia and sodium periodate, two other working curves of aloin and aloe-emodin were also made. The samples were prepared under the same conditions. The two values of absorbance were obtained. Based on four linear equations, the contents of aloin and aloe-emodin were obtained. The detection limits of aloin and aloe-emodin were 0.0024 and 0.0006 mg/mL respectively. The relative standard deviation was less than 7% and the recovery was 99%～101%.

关 键 词：芦荟甙 芦荟大黄素 定量分析 分光光度法 

分 类 号：O657.23[理学—分析化学]