中药外敷对支气管哮喘β受体及T淋巴细胞的影响  

Effect of external application of Chinese herbs on β receptor and Τ lymphocyte subsets in patients with brochial asthma

作　　者：李鹤[1] 沈惠风[1] 闵亮[1] 卢锦花[1] 秦亮甫[1] 夏宗勤[2] 胡雅儿[2] 

机构地区：[1]上海第二医科大学仁济医院中医科,上海200001 [2]上海第二医科大学基础医学院核医学教研室

出　　处：《上海第二医科大学学报》2004年第B11期53-55,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市科学技术发展基金 (9840 1 90 2 9)资助项目

摘　　要：目的中药外敷前后对哮喘患者β受体及Τ淋巴细胞的影响。方法随机选择门诊支气管哮喘患者 30例 ,每周 2次予中药外敷 30min～ 1h不等 ,共计 18次 ,治疗前后抽取肝素血 5mL ,放射配基竞争结合法比较 β受体含量 ;流式细胞仪对外周血CD4 + 、CD8+ Τ淋巴细胞定性分析。结果 30例患者经中药外敷后外周血β受体含量明显增高 (P <0 .0 1) ;治疗后CD8+ Τ淋巴细胞较治疗前百分比提高 (P <0 .0 5 ) ,CD4 + 、CD8+ Τ淋巴细胞比值降低。结论提高β受体含量是中药外敷治疗支气管哮喘患的重要机制之一 ,而提高CD8+ Τ淋巴细胞百分比和降低CD4 + 、CD8+ Τ淋巴细胞比值是中药外敷治疗支气管哮喘患的又一重要机制。Objective To study the effect of external application of Chinese herbs on β receptor and Τ lymphocyte subsets in patients with bronchial asthma before and after the treatment. Methods The subject is 30 randomly chosen cases of bronchial asthma from the outpatient service. The mixing herbs were put on the patients back by application twice a week and total is 18 times. 5 mL heparinized venous blood was extracted before and after treatment to compare the content of β receptor and to qualitatively analyze peripheral blood CD4 +,CD8 +Τ lymphocyte by radioligand binding assay and flow cytomejry. Results The content of peripheral blood βreceptor rose significantly after treatment by Chinese herbs application (P<0.01). The percentage of CD8 +Τ lymphocyte rose (P<0.05) and the ratio of CD4 +/CD8 + dropped. Conclusion One of the most important mechanisms is to increase the content of βreceptor in treatment bronchial asthma by Chinese herbs application, and of course, it is also very important to rise the percentage of CD8 +Τ lymphocyte and reduce the ratio of CD4 +/CD8 +.

关 键 词：T淋巴细胞 Β受体 支气管哮喘 CD8^+ 中药外敷治疗 CD4^+ 患者 竞争结合 流式细胞仪 定性分析 

分 类 号：R244.9[医药卫生—针灸推拿学]