黑胸大蠊体部和粪中变应原的交叉性与哮喘的关系  

Study on allergenic cross-reactivity between whole body and feces of periplaneta fuliginosa and its relationship to bronchial asthma

作　　者：许以平[1] 张君[1] 姚苏杭[1] 朱丽君[1] 王菁兰[1] 曹玲仙[1] 

机构地区：[1]上海第二医科大学仁济医院过敏疾病防治中心,上海200001

出　　处：《上海第二医科大学学报》2004年第B11期1-3,8,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市高等学校科技基金 (98B1 4 )资助项目

摘　　要：目的探讨黑胸大蠊体部浸液 (WBE)和粪浸液 (FE)的变应原交叉性及其与哮喘的关系。方法支气管哮喘患者和健康人各 30例 ,分别以WBE和FE作人嗜碱粒细胞脱颗粒试验 (HBDT)和sIgE检测 (ELISA法 )。采用SDS PAGE和免疫印迹试验分析和确定变应原的组分 ,进而用ELISA和免疫印迹抑制试验观察WBE和FE的交叉性。结果 1.哮喘组HBDT和sIgE的阳性率均明显高于健康组 (P <0 .0 5 ) ;2 .SDS PAGE显示 ,WBE至少有 30条可分辨的蛋白条带 ,而FE有 15条蛋白带 ,其中至少有 8条(97.4、79.5、72、6 6、4 3、4 1、36、2 4kd)与WBE处于相同的位置 ,免疫印迹试验显示 ,9例sIgE呈阳性的哮喘患者中 88.9% (8/ 9)出现阳性条带 ,其中 72、36kd较常见 ;3.将WBE或FE预先与阳性血清作用后可抑制阳性血清的ELISA和免疫印迹试验的阳性反应。结论黑胸大蠊的体部和粪便中具有相似的变应原性 ,与哮喘的发病有关 ,其主要变应原组分为 72、36kd。Objective To study on allergenic cross-reactivity between whole body extract (WBE) and fecal extract (FE) of Periplaneta Fuliginosa, and its relationship to asthma. Methods Human basophil degranulation test (HBDT) and ELISA were evaluated and compared in 30 healthy subjects and 30 asthmatics, using WBE and FE respectively. Furthermore, the allergenic components were analysed and determined by SDS-PAGE and western blotting, The cross-reactivity between WBE and FE was observed by inhibition of ELISA and western blotting. Results (1) The positive rates for WBE-HBDT, WBE-sIgE were 40% and 33.33% respectively, in asthmatic group, much higher than that of 6.67% and 0% in healthy group (P<0.05). The positive rates for FE-HBDT, FE-sIgE were 45% and 33.33% respectively, much higher than that of 10% and 0% in healthy group (P<0.05). The strict concordance between WBE and FE was seen. (2) More than 30 bands for WBE, 15 bands for FE could be differentiated by SDS-PAGE. There are at least 8 bands (97.4, 79.5, 72, 66, 43, 41, 36, 24 kd) were located in same place. Among 9 asthmatics with positive sIgE 88.9% (8/9) showed positive bands, by western blotting in which 72, 36 kd are much common. (3) The positive results of ELISA and western blotting could turn to negative after incubation of positive serum with WBE or FE. Conclusion The whole body and feces of periplaneta fuliginosa, containing similar allergenic activity, are both related to the pathogenesis of bronchial asthma. The molecule weight of major allergens are 72, 36 kd.

关 键 词：变应原 哮喘 黑胸大蠊 体部 免疫印迹试验 ELISA 蛋白 阳性血清 SDS-PAGE 抑制试验 

分 类 号：R562.25[医药卫生—呼吸系统]