银翘解毒丸中金银花药材HPLC-UV鉴定及绿原酸含量测定  

HPLC-UV identification of Flos Lonicerae and determination of chlorogenic acid in Yinqiao jiedu Pills

作　　者：柳仁民[1] 邓爱霞[1] 

机构地区：[1]聊城大学化学化工学院,山东聊城252059

出　　处：《中成药》2004年第12期999-1002,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立银翘解毒丸(金银花,连翘,甘草,薄荷等)中金银花药材的鉴别和绿原酸含量测定的方法。方法采用YWGC18色谱柱;甲醇10%的磷酸氢二钠(冰醋酸调pH值为2.7)为流动相进行梯度洗脱;检测波长为326nm;流速为1.0mL·min-1;柱温为室温。结果HPLCUV法定性鉴别制剂中金银花药材;绿原酸在5.0～60μg·mL-1的范围内呈良好线性关系(r=0.9999),平均回收率为98.71%(RSD=1.57%)。结论本法操作简单,准确,重现性好,可用于银翘解毒丸的质量控制。AIM: To set up identification of Flos Lonicerae and determination of chlorogenic acid in Yingqiao jiedu Pills (Flos Lonicerae, Fructus Forsythiae, Radix Glycyrrhizae, Herba Menthae, etc.) by HPLC. METHODS: A YWG C 18 column was used with the mobile phase consisted of CH 3OH 10%Na 2HPO 4 (adjusting pH to 2.7 with HAc) as gradient elution. The flow rate was 1.0mL·min -1 , and the detection wavelength was at 326nm. RESULTS: Flos Lonicerae in Yinqiaojiedu Pills was identified by HPLC UV. The linear range of chlorogenic acid was 5.0～60μg·mL -1 ( r =0.9999). The average recovery was 98.71% with RSD of 1.55% ( n =5). CONCLUSION: The method is simple, accurate and rapid with good reproducibility. It can be used for the quality control of Flos Lonicerae in Yinqiaojiedu Pills.

关 键 词：HPLC 银翘解毒丸 金银花 绿原酸 

分 类 号：R927.2[医药卫生—药学]