黄连配方颗粒质量标准研究  

Quality Standard for Huanglian Despensing Granules

作　　者：李希[1] 罗先本[1] 谢守德[1] 张嵩[1] 

机构地区：[1]四川省中医药研究院中医研究所,四川成都610031

出　　处：《中成药》2004年第12期1010-1012,共3页Chinese Traditional Patent Medicine

摘　　要：目的建立黄连配方颗粒质量标准。方法采用薄层色谱法,对黄连颗粒中盐酸小檗碱、盐酸药根碱、盐酸巴马汀进行了薄层定性鉴别;采用高效液相色谱法对其中的盐酸小檗碱、盐酸药根碱进行了含量测定。结果盐酸小檗碱在0.17076～0.838μg间线性关系良好,r=0.99996,平均回收率为98.82%,RSD为1.702%;盐酸药根碱在0.11796～0.5898μg间线性关系良好,r=0.99959,平均回收率为98.4%,RSD为1.312%。在TLC图谱中可检出黄连的特征斑点。结论本法操作简便、准确、重现性好,能够控制黄连配方颗粒的质量。AIM: To establish the quality standard of Huanglian Despensiing Granules (Rhizoma Coptidis). METHODS: TLC identifications of berberine hydrochloride, jateorrhizine hydrochloride and palmatine hydrochloride in Rhizoma Coptidis carried out. The contents of berberine hydrochloride and jateorrhizine hydrochloride were determined by means of HPLC. RESULTS: The linear ranges of berberine hydrochloride and jateorrhizine hydrochloride were 0.17076～0.838μg( r =0.99996), and 0.11796～0.5898μg( r =0.9959), respectively their average recoveries were 98.82 ( RSD =1.702) and 98.4 ( RSD =1.312), respectively. The characteristic spots of Rhizoma Coptidis could be detected by TLC. CONCLUSION: The methods reported in this paper are easy to operate, and can provide accurate and reproducible results, thus are able to control the quality of the Huanglian Despensing Granules.

关 键 词：黄连配方颗粒 TLC HPLC 盐酸小檗碱 盐酸药根碱 

分 类 号：R927.2[医药卫生—药学]