一株烟酸羟基化转化菌株的筛选和鉴定  

Screening and identification of a strain for hydroxylation of nicotinic acid

作　　者：陆伟宏[1] 徐莉[1] 戴亦军[1] 袁生[1] 

机构地区：[1]南京师范大学生命科学学院江苏省资源生物技术重点实验室

出　　处：《微生物学报》2005年第1期6-9,共4页Acta Microbiologica Sinica

基　　金：国家"十五"科技攻关项目 ( 2 0 0 1BA3 0 8A0 5_0 2 )~~

摘　　要：从南京地区的土壤中筛选到一株高效转化烟酸为 6_羟基烟酸的菌株NA_1。形态及生理生化特征测定结果表明 ,NA_1菌株与假单胞菌属 (Pseudomonas)中的恶臭假单胞菌 (P .putida)种的特征基本一致。测定了该菌株的16SrDNA序列并根据 16SrDNA构建了系统发育树 ;在系统发育树中 ,NA_1菌株与恶臭假单胞菌形成一个类群 ,序列同源性为 99%。Strain screening was conducted on a large scale in order to develop a biotransforming process for 6-hydroxynicotinic acid production. A strain named as NA-1 was isolated from soil which could transform nicotinic acid into a new product showed by HPLC. The new product was identified as 6-hydroxynicotinic acid by MS and 1H-NMR. The morphology, physiological and biochemical characteristics of strain NA-1 were studied and showed that characteristics of strain NA-1 were essentially consistent with Pseudomonas putida. The analysis of 16S rDNA sequence from strain NA-1 suggested that strain NA-1 was clustered together with P. putida in phylogenetic tree and the sequence identity between stain NA-1 and Pseudomonas putida was 99%. So strain NA-1 was identified to P. putida.

关 键 词：烟酸 6-羟基烟酸 生物转化 恶臭假单胞菌 

分 类 号：Q939.97[生物学—微生物学]