圈卷产色链霉菌分化相关基因-samR调控的初步研究  

Preliminary study on autoregulation of samR involved in development and differentiation of Streptomyces ansochromogenes

作　　者：杨艳玲[1] 杨海花[1] 谭华荣[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》2005年第1期10-13,i002,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金 ( 3 0 170 5 10 0 );国家"863计划"( 2 0 0 4AA2 14 13 0)~~

摘　　要：在野生型的圈卷产色链霉菌中 ,增加单个或多个拷贝数的samR ,观察该基因对野生株表型和形态的影响。结果表明 ,samR的拷贝数的增加会使孢子形成提前 ,但是增加单拷贝的菌株与增加多拷贝的菌株所表现的增效作用相同。在大肠杆菌中对samR进行了GST融合表达和纯化 ,通过凝胶阻滞实验证明 ,SamR蛋白可与其上游的调控区特异性结合。The previous result showed that samR plays an important role in the development progress of Streptomyces ansochromogenes. It was reported that the differentiation progress of S. ansochromogenes was accelerated by a recombinant plasmid containing anextra copy of samR gene. However, the differentiation progress of S. ansochromogenes was not further accelerated by a multicopy plasmid containing samR gene. Electrophoresis mobility shift assay (EMSA) demonstrated that SamR binds to its own promoter region specifically. All these results hint that samR is an autoregulatory gene in Streptomyces ansochromogenes.

关 键 词：链霉菌分化 samR基因 自调控 

分 类 号：Q933[生物学—微生物学]